Abstract

MARTINEZ-MAZA, Nayely Teresa et al. Molecular analysis of a polypeptide of the RmS-17 protein for the development of a vaccine against Rhipicephalus microplus ticks in Mexico. Ecosistemas y recur. agropecuarios [online]. 2020, vol.7, n.3, e2537.  Epub Nov 08, 2021. ISSN 2007-901X.  https://doi.org/10.19136/era.a7n3.2537.

The aim of this work was to analyze a RmS-17 polypeptide from Rhipicephalus microplus strains and isolates from different states of Mexico. in silico analysis of the amino acid sequence was performed in order to select a region of 145 amino acids. Subsequently, a pair of specific oligonucleotides was designed to amplify the fragment of interest in each strain and isolate, obtaining a 435 bp amplicon. The products were cloned into the pCR® 4-TOPO® vector and the plasmids transformed into the competent E. coli TOP10® cells. Positive clones were selected for sequencing purposes. The results showed an identity percentage of 99.08% for the reference strain “Media Joya” with respect to the previously reported strain in Brazil (KC990116.1). The strains “Vargas”, “Hybrid”, “Huastecas” and the isolates from Campeche, Chiapas, Hidalgo, Morelos and Guerrero an identity and similarity percentage of 100% was observed. These results demonstrate a high degree of conservation in the sequence of the RmS-17 polypeptide and suggests that it is appropriate to carry out the development of an immunogen that will be useful to control R. microplus infestations in several geographical areas of Mexico.

Keywords : Ticks; immunogen; Mexico; R. microplus; RmS-17.

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