Abstract

MARTINEZ-CANTO, Orlando José; CRISTOBAL-ALEJO, Jairo; TUN-SUAREZ, José María  and  REYES-RAMIREZ, Arturo. Detection of Epl1 and Sm1 genes in Trichoderma spp. antagonists againstphytopatogenic fungi. Ecosistemas y recur. agropecuarios [online]. 2021, vol.8, n.2, e2791.  Epub Aug 12, 2021. ISSN 2007-901X.  https://doi.org/10.19136/era.a8n2.2791.

Trichoderma spp. are used as a method of biocontrol of phytopathogenic that cause agricultural losses. The objective of this work was to identify nine Trichoderma strains, determine the antagonistic capacity against Fusarium oxysporum and Corynespora cassiicola, as well as the genes that encode the elicitors Sm1 and Epl1 of systemic resistance in plants. The antagonistic capacity against phytopathogens was determined through dual confrontations. The molecular identification corresponded to T. asperellum (19-31), T. erinaceum (10-15), T. ghanense (21-39) and T. harzianum (18-31, 05E-60, 20-35, 13-01, 01-01, 29-54). Strains 10-15, 19-31 and 05E-60 showed greater mycelial inhibition capacity against F. oxysporum, while the strains 32-09 and 27-08 against C. cassiicola.

The volatile metabolites of 27-08, 21-39 and 05E-60 strains inhibited the growth of F. oxysporum. The presence of the Epl1 genes were identified in 19-31 and 10-15 strains, and Sm1 in 27-08 and 32-09 strains.

Keywords : Corynespora cassiicola; Fusarium oxysporum; micelial inhibition; volatile metabolites.

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