Abstract

PEREZ-ALVAREZ, Sandra et al. Biochemical and molecular activity of oxidative stress enzymes in tomato plants growth with lead. Ecosistemas y recur. agropecuarios [online]. 2021, vol.8, n.3, e3163.  Epub Oct 31, 2022. ISSN 2007-901X.  https://doi.org/10.19136/era.a8n3.3163.

The aim of this research was to study the activity at biochemical and molecular level of oxidative stress enzymes in tomato plants cv. Mariela grown with different concentrations of lead (0, 50 and 100 mg kg-1 of PbAc2). For the biochemical activity, the enzymes superoxide dismutase (SOD) and glutathione reductase (GR) were selected; in addition, the protein concentration was determined. Molecular activity was studied by real-time PCR for three genes: SOD, isoflavone reductase (IFR), and transcriptionally controlled tumor protein (TCTP). In leaves and stems there were significant differences in proteins compared to the control. The GR showed that in leaves the highest activity was achieved at 50 mg kg-1 of PbAc2, while in stems there were differences with the control. In leaves and stems, the lowest SOD activity was when using PbAc2. The expression of the three genes in leaves was higher with PbAc2 and in stems it varied.

Keywords : Soil contamination; oxidative stress; heavy metals; Solanum lycopersicum L.; plant toxicity.

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