Abstract

BENITEZ-GONZALEZ, Edgar et al. Comparative evaluation of two methods of spermatic recovery of post-mortem bovine epididymis. Abanico vet [online]. 2018, vol.8, n.1, pp.59-74. ISSN 2448-6132.  https://doi.org/10.21929/abavet2018.81.6.

Determining the appropriate method to recover epididymal spermatozoa in bovines at different post-mortem recovery times was the objective of the present study. Testicles of 50 bulls of different breeds and ages, in good sanitary condition, were obtained after the slaughter in the animal commercialization square of Loja, Ecuador; evaluating two methods of seminal collection: retrograde flushing and slicing. The testes/epididymis were transported in 0.9% sodium chloride solution and stored for 2, 4, 8, 12 and 24 hours at 5 °C, evaluating sperm quality in relation to the time elapsed from slaughter to sperm production in the laboratory. The evaluated parameters were: mass motility, individual motility, percentage of viable sperm, and normal. In the analysis of the results descriptive statistics were used and for the variance analysis Tukey test were used to determine the statistical differences between averages. The epididymal spermatozoa showed: mass motility 60.4% ± 4.75; individual motility 50.7% ± 4.75; vitality 60.6% ± 3.85; abnormalities 8.78% ± 1.4; there is no significant difference (p>0.05) between protocols, getting average volumes of 2.2±05 ml, concentration of 63.08% ± 2.05, the percentage of living sperm was more when the method of retrograde wash was used. It is concluded that is possible collect living sperm from the post-mortem epididymis bull’s tail, being its vitality directly proportional to the storage time.

Keywords : Epididymal; spermatic quality; retrograde washing; slicing.