Abstract

DELGADILLO-RUIZ, Lucía et al. Use of plant extracts in in vitro ruminal fermentation. Abanico vet [online]. 2019, vol.9, e917.  Epub Mar 05, 2021. ISSN 2448-6132.  https://doi.org/10.21929/abavet2019.917.

The objective of this work was to obtain and characterize the extracts of Larrea tridentata, Origanum vulgare, Artemisa ludoviciana and Ruta graveolens for use in ruminal digestibility in vitro and to quantify their production of gas, volatile fatty acids and methane. The chemical composition of the extracts was determined by gas chromatography and ruminal fluid was used for in vitro gas production. The determination of volatile fatty acids was carried out by gas chromatography. Methane was inferred with the concentration of volatile fatty acids. For the chemical composition, the compounds were identified: terpinen, limonene, linalool, thymol and carvacrol. In digestibility, the lowest concentration of gas produced at a volume of 100 μL of extract was L. tridentata and methane is reported 1.4 times more than the control of alfalfa (0.514 mL g-1). For the concentration of 330 μL of the O. vulgaris extract, it showed inhibition of methane with respect to the control of alfalfa. It is concluded that the extract four of O. vulgaris was the one that presented a better digestibility with respect to the production of gas, AGV (volatile fatty acids) and lower concentration of methane with respect to the control.

Keywords : Plant extracts; Volatile fatty acids and Methane.