Abstract

LOEZA-CONCHA, Henry et al. Effect of egg yolk on sperm cryopreservation of drone (Apis mellifera). Abanico vet [online]. 2019, vol.9, e921.  Epub Mar 05, 2021. ISSN 2448-6132.  https://doi.org/10.21929/abavet2019.921.

The aim of this study was to evaluate the effect of whole yolk (WEY) and centrifuged yolk (CEY) and its interaction with dimethylsulfoxide (DMSO) and glycerol, on the cryopreservation of drone semen. Six aliquots of semen were obtained, which were divided into four treatments: T1 (CEY+ glycerol), T2 (CEY + DMSO), T3 (WEY + glycerol) and T4 (WEY + DMSO). The diluted samples were evaluated after 10 minutes of incubation at 37 °C and after thawing. Motility was evaluated subjectively (scale 4 to 0), membrane integrity (Host test) and viability (IP/ SYBR-14). The results were analyzed with ANOVA. At the time of dilution, T1 was the highest percentage of motility, viability and integrity of the membrane presented (grade 4, 95.75% ± 1.20, 89.75% ± 0.42, respectively), after thawing the T2 was the highest percentage presented (grade 2, 59.10% ± 1.44, 84.35% ± 0.72, respectively). The results showed that T1 was the one that obtained the best sperm parameters at the time of dilution, while, after thawing, T2 was the diluent that best cryopreserved sperm samples from drone.

Keywords : Egg yolk; cryopreservation; semen; drone.

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