Abstract

SILVA-CONTRERAS, Amador et al. Africanization of colonies of Apis mellifera L. (Hymenoptera:Apidae), present in the mitochondrial DNA. Abanico vet [online]. 2019, vol.9, e922.  Epub July 30, 2021. ISSN 2448-6132.  https://doi.org/10.21929/abavet2019.922.

Mexican beekeeping is an activity associated with producers of low income whose products are intended for export. The present study was conducted to assess the degree of africanization of the commercial apiaries through the presence of African genes in deoxyribonucleic acid (DNA) mitochondrial of honeybees (Apis mellifera L.). We collected bees from commercial apiaries that were kept in alcohol ethanol until the DNA extraction. The Retrieved DNA was frozen at - 20 °C until use. 50 µg that was digested PCR product were used for the PCR-RFLP and incubated for 16 hours and the products of digestion were separated by electrophoresis in an agarose 1% solution TBE 1X gel. The staining of the gels was performed with ethidium bromide. A pattern of two fragments (194 and 291 pb) was visualized corresponding to the mitotype European (E) and a fragment only undigested 485 PB which corresponds to the African mitotype. The population of Hardy-Weinberg are not in balance, there is deficit of heterozygotes.

Keywords : Africanization; PCR; Norther Mexico.

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