Abstract

RODRIGUEZ-GUTIERREZ, Itzel et al. Antioxidant effect of Trolox and Crocina on the cryopreservation of Pelibuey ovine semen. Abanico vet [online]. 2020, vol.10, e2.  Epub June 30, 2020. ISSN 2448-6132.  https://doi.org/10.21929/abavet2020.4.

The objective of the study was to evaluate the effect of the antioxidants Trolox and Crocina in the freezing diluent on the cryopreservation of Pelibuey ovine semen. The ejaculates obtained were mixed, divided and frozen in 3 treatments: T1: 1mM Trolox; T2: 1mM Crocina; TT: Control (without antioxidant). At 0 hours (defrosting) and 6 hours at 37 °C, total (MT) and progressive (MP) motility, sperm viability, mitochondrial activity, integrity of acrosomes and tail membrane were analyzed (Host) The data was analyzed with an ANOVA for a completely randomized design and a Tukey test for the comparison of means. In neither of the two hours were significant differences found in the treatments (P> 0.05). However, from 0 to 6h, MT behaved similarly in T1 and T2, but not in TT (P <0.01), on the contrary, the integrity of acrosomes in all three treatments was similar (P> 0.05). MP, viability, mitochondrial activity and Host decreased over time in a similar way in all three treatments. The antioxidants Trolox and Crocina at 1mM in the freezing diluent, allow to maintain the MT and the integrity of the acrosomes for 6 h of incubation at 37 °C.

Keywords : Free radicals; diluent; sperm and freezing.

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