Abstract

ANTONIO-IRINEO, Nora et al. Preliminary study on the in vitro inhibition of gastrointestinal nematodes from sheep with aqueous extracts of forage plants. Abanico vet [online]. 2021, vol.11, e402.  Epub May 21, 2021. ISSN 2448-6132.  https://doi.org/10.21929/abavet2021.10.

The presence of gastrointestinal nematodes (GIN) in sheep is a low productivity cause. This study aimed to determine the in vitro efficiency of Gliricidia sepium, Leucaena leucocephala, Guazuma ulmifolia, and Bursera simaruba aqueous extracts at three different doses (0.75, 1.00, and 1.25 mL), inhibiting the egg hatching of GIN from sheep. The inhibition efficiency in egg hatching (IEH), larval identification, and its genera proportion were evaluated. Analysis of data was performed using Chi-square tests and analysis of variance. The four extracts obtained an IEH of 50%, being similar (p > 0.05) between them. The 1.25 mL dose and its combinations of Leucaena leucocephala and Gliricidia sepium at 1.25 mL dose obtained the highest efficiency (68.0, 85.0, and 77.0%, respectively). Five genera of larva were identified (Haemonchus spp, Trichostrongylus spp, Oesophagostomum spp, Cooperia spp, and Nematodirus spp). The highest prevalence (p ≤ 0.05) was obtained by Haemonchus spp (58.0%). According to the results, the four aqueous extracts exhibited ovicidal activity (GIN). However, the 1.25 mL dose and the Leucaena leucocephala, Gliricidia sepium extracts at 1.25 mL doses were the most effective.

Keywords : Gastrointestinal parasites; anthelmintic plants; egg hatching.