Abstract

TUN-MOO, Maximiliano et al. Inclusion of soybean oil in the diet on cryopreservation of ram semen. Abanico vet [online]. 2022, vol.12, e2020-13.  Epub June 23, 2023. ISSN 2448-6132.  https://doi.org/10.21929/abavet2022.23.

The objetive was to evaluate the effect of soybean oil (SO) supplemented diet on the cryopreservation of Pelibuey ram semen. Twenty-seven rams were divided three treatments (n= 9): T1: 0% SO (control); T2: 3% SO and T3: 6% SO, for a period of 60 days. The frozen-thawed semen was assessed for sperm motility parameters, plasma membrane integrity, mitochondrial activity, acrosome integrity and tail membrane integrity (Host). The results were analyzed by ANOVA, followed by Tukey's comparison test. T1 and T3 were similar and superior (P<0.05) to T2 in total motility and progressive motility. Mean velocity and acrosome integrity in T1 were higher (P<0.05) compared to T2 and T3. Viability in T1 was similar to that of T2 and T3, however, T2 and T3 were different (P<0.01). Mitochondrial activity in T3 was higher, compared to T1 and T2 (P<0.05). No differences (P>0.05) were found in the other motility parameters and in host. The inclusion of soybean oil in the diet did not improve the cryopreservation of ram semen, although the inclusion of 6% soybean oil in the diet increased mitochondrial activity.

Keywords : fatty acid; spermatozoa; frozen-thawed.