SciELO - Scientific Electronic Library Online

 
vol.6 número7Eficiencia agronómica de fertilización al suelo de macro nutrimentos en híbridos de maízTendencias y variabilidad de índices de cambio climático: enfoque agrícola en dos regiones de México índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay artículos similaresSimilares en SciELO

Compartir


Revista mexicana de ciencias agrícolas

versión impresa ISSN 2007-0934

Resumen

RANGEL-ESTRADA, Sandra Eloísa et al. In vitro regeneration of poinsettia hybrids via organogenesis. Rev. Mex. Cienc. Agríc [online]. 2015, vol.6, n.7, pp.1571-1585. ISSN 2007-0934.

Poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch) is an ornamental plant native of Mexico prized for their striking bracts and widely cultivated in many countries. Globally it is considered an icon of Christmas. The different in vitro plant tissue culture techniques have contributed to the genetic improvement of various crops and other species. In this study a protocol for in vitro propagation of poinsettia hybrids 'Zacatepec 10' and 'Zacatepec 48' from the Ornamental Plant Breeding program of INIFAP, from the experimental field of Zacatepec was developed. The induction of organogenesis was achieved through the activation of axillary buds from nodal segments obtained from mother plants two months old. Incubation of explants in MS medium (1962) supplemented with 8.8 μM BA for eight weeks, induced the activation of axillary bud and 3.9 new hybrid shoots in 'Zacatepec 10' and 2.5 in 'Zacatepec 48' with average size 1.02 cm. Shoot multiplication was effective with 4.4 μM BA combined with 2.2 μM CIN added with 0.5 μM of AIA, but increased substantially with 0.23 μM TDZ; 12.1 shoots in Zacatepec 10' and 11.6 in' Zacatepec 48 'after eight weeks of culture. In rooting were obtained on average 5.8 roots of 5.9 cm length in culture medium with half salt concentration added with 5.6 μM AIA after six weeks. Acclimatization of 85% of the plants of the two hybrids was achieved with a mix of peat and perlite (1: 1) after six weeks of planting. This protocol will be implemented for clonal propagation of new poinsettia hybrids that have been generated in Mexico and need to multiply, because conventional propagation limits the amount of new plants and some have few specimens.

Palabras llave : Euphorbia pulcherrima; genetic improvement; micropropagation; Nodal segments.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons