Servicios Personalizados
Revista
Articulo
Indicadores
- Citado por SciELO
- Accesos
Links relacionados
- Similares en SciELO
Compartir
Salud Pública de México
versión impresa ISSN 0036-3634
Resumen
HERNANDEZ-PEDRO, Norma et al. Mutational profile by targeted next generation sequencing of non-small cell lung cancer in the Mexican population. Salud pública Méx [online]. 2019, vol.61, n.3, pp.308-317. Epub 03-Mar-2020. ISSN 0036-3634. https://doi.org/10.21149/10113.
Objective:
Targeted next-generation sequencing (t-NGS) has revolutionized clinical diagnosis allowing multiplexed detection of genomic alterations. This study evaluated the profile of somatic mutations by t-NGS in Mexican patients with non-small cell lung cancer (NSCLC).
Materials and methods:
Genomic DNA was extracted from 90 lung adenocarcinomas and sequences were generated for a panel of 48 cancer genes. Epidermal Growth Factor Receptor (EGFR) mutations were detected in parallel by quantitative PCR.
Results:
The mutational profile of NSCLC revealed alterations in 27 genes, where TP53 (47.8%) and EGFR (36.7%) exhibited the highest mutation rates. EGFR Q787 mutations were present in 14 cases (15.6%), 10 cases had exon 19 deletions (11.1%), seven cases had L858R (7.8%). The mutational frequency for genes like EGFR, MET, HNF1A, HER2 and GUSB was different compared to caucasian population.
Conclusion:
t-NGS improved NSCLC treatments efficacy due to its sensitivity and specificity. A distinct pattern of somatic mutations was found in Mexican population.
Palabras llave : mutational analysis, DNA; adenocarcinoma; lung; DNA sequencing.