Resumen

CRUZ MUNOZ, Rocío et al. Pycnoporus sanguineus pigment production on solid media. Agrociencia [online]. 2015, vol.49, n.4, pp.347-359. ISSN 2521-9766.

Pycnoporus is a representative genus of saprophytic homobasidiomycetes that has lignocellulytic potential. The secondary metabolites of this fungus have gained importance in the search for antiviral, antioxidant, antifungal and antibacterial substances. Therefore, the objective of this study was to evaluate P. sanguineus growth and production of pigment in four solid media. Basidiocarps from P. sanguineus obtained from decomposing plant material of casuarina (H1) and mango (H2) trees were used. The experimental design was completely randomized with a factorial arrangement of treatments in which the factors and levels were: 1) culture medium (potato dextrose agar, malt extract agar, whole-wheat flour agar, casuarina extract agar and mango extract agar) and 2) pH (7.0, 8.0 and 9.0). The 30-day study determined the fungus growing conditions and production of orange pigment. The response variables were colony diameter (mm), specific growth rate (h^-1), concentration of orange pigment (mg mL^-1) and quantity of extracted pigment (mg). With the data, an ANOVA was performed and the means were compared by orthogonal contrasts (p≤0.05, 0.01, 0.001). The optimum conditions for growth of the H1 isolate were casuarina extract agar at pH 7.0 and 9.0, whereas for the H2 isolate, they were mango extract agar at pH 7.0. The fastest growth rate in both cases occurred in the culture mediums of extracts from which they were isolated. The highest concentrations of pigment were obtained in potato dextrose agar and malt extract agar, 0.0175 and 0.0168 mg mL^-1 for H1 and H2 (p≤0.05), respectively. The largest quantity of pigment was extracted in potato dextrose agar and mango extract agar pH=7.0, 55.6 and 57.1 mg, for H1 and H2, respectively (p≤0.05).

Palabras llave : Pycnoporus sanguineus; biological activity; Casuarina equisetifolia; Mangifera indica; biocolorants; phenoxacine.