Resumen

LINARES-HOLGUIN, O. Omer; SANCHEZ-PENA, Pedro  y  MOLINA-FREANER, Francisco. Genetic diversity of the chloroplast (TrnL-F) region among populations of Pholisma culiacanum Y. Agrociencia [online]. 2016, vol.50, n.7, pp.799-809. ISSN 2521-9766.

Pholisma culiacanum is a root holoparasitic plant, endemic to the Mexican states of Sinaloa and Sonora that has cultural, nutritious, biological, and evolutionary importance. This plant is an adequate agricultural model as an alternative source of nutrients and as a tool for understanding photosynthesis’ molecular changes, from autotrophism to parasitism. The aim of this study was to analyze the genetic diversity of seven populations of P. culiacanum in Sinaloa and Sonora. Sequences from a chloroplast DNA (TrnL-F) fragment were analyzed in 70 samples, in order to calculate the genetic variation within (86.51 %) and between (13.49 %) populations. Correlation and simple linear regression analysis were performed for the genetic and geographic distances data. Haplotype and nucleotide diversity was detected at 0.85 and 0.27; 11 haplotypes were identified as well. The relationship between genetic and geographic distances was not significant (p>0.05); therefore, no evidence of isolation due to distance was found. Based on the analyzed information, areas of in situ exploitation and genetic resources conservation are proposed.

Palabras llave : Holoparasitic; Pholisma culiacanum; genetic diversity; chloroplast; TrnL-F.

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