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Revista odontológica mexicana
versión impresa ISSN 1870-199X
Rev. Odont. Mex vol.10 no.4 Ciudad de México dic. 2006
Trabajos originales
Análisis microscópico de la adherencia de Candida albicans in vitro sobre resina acrílica utilizada para bases de dentaduras procesada con tres diferentes técnicas
Microscopic in vitro analysis of the Candida albicans adherence in acrylic resin used to process dentures with three different techniques
* Laboratorio de Prostodoncia Total. Facultad de Odontología, UNAM.
§ Departamento de Biología Celular y Tisular. Facultad de Medicina, UNAM.
II Laboratorio de Micología Médica, Instituto Nacional de Enfermedades Respiratorias, INER.
Antecedentes
La estomatitis protésica está asociada a la adherencia y colonización de Candida albicans a la base de las dentaduras y a otros cofactores, como un pH ácido, ingesta aumentada de carbohidratos, diversas enfermedades sistémicas y tratamientos farmacológicos.
Objetivo
Analizar mediante microscopia electrónica de barrido (MEB), la adherencia de Candida albicans in vitro, sobre muestras de resina acrilica de polimetilmetacrilato usadas para bases de dentaduras, las cuales fueron procesadas con tres diferentes técnicas.
Diseño del estudio
Se elaboraron 18 muestras de resina acrílica con tres técnicas de procesado, en las cuales se inoculó Candida albicans in vitro durante 24 у 48 h; dichas muestras se observaron mediante MEB de donde se obtuvieron fotografías a un aumento de 600x, 1,500x y 6,000x, las cuales se utilizaron como panorámica, conteo de blastoconidios y detalle, respectivamente.
Resultados
La resina elaborada con termo-polimerizado con inyección (TPI) presenta poros de 1 a 2 mieras de diámetro, a 24 h de inoculación se observan blastoconidios y formación de seudomicelio mientras a 48 h hay numerosas células en gemación. En el auto y termo-polimerizado con inyección (ATPI) hay poros de .5 a 5 mieras asi como grietas de 100 mieras de largo, a 24 h los blastoconidios tienen forma irregular con colapso en su superficie en tanto a 48 h hay seudomice-Nos, gemación y las células siguen presentando irregularidades. En el polimerizado mediante microondas (PM) hay poros de 1 a 5 mieras, a 24 h hay seudomicelios y células en gemación, tanto que a 48 h la formación de seudomicelios es mayor, así como la gemación.
Conclusiones
La resina acrílica que se elaboró con la técnica de termo-polimerizado con inyección presenta la superficie con menos defectos de las tres técnicas estudiadas y no hay diferencia significativa en el número de células adheridas de 24 a 48 h de inoculación.
Palabras clave: Candida albicans; adherencia; polimetilmetacrilato; Estomatitis protésica; Microscopia electrónica de barrido
Antecedents
Prosthetic stomatitis is associated to the adherent and colonization of Candida albicans to denture bases and other cofactors such as acidic pH, high carbohidrate diet, systemic diseases as well as pharmacological treatments.
Objective
To analyze Candida albicans adherence in vitro on different samples of polymetacrilate acrylic resin used for denture base, processed with three different techniques using scanning electron microscopy (SEM).
Study design
18 different samples of acrylic resin were made using three different techniques. Candida albicans was inoculated in vitro for 24 and 48 hours. These samples were observed under the microscope, getting pictures of them at 600x, 1,500x and 6,000x magnification.
Results
The resin elaborated with thermopolimerization injection (TPI) presents pores of 1-2 microns in diameter. At 24 hours after inoculation blastoconidios can be observed, as well as the formation of pseudomycelium while at 48 there are numerous cells in gemmation. In auto and termopolimerization injection (ATPI) there are pores measuring .5-5 microns in diameter as well as cracks with 100 microns in length. At 24 hours the blastoconidios have irregular shapes while at 48 hours there are pseudomycelium, gemmation and cells present irregularities. In microwave polimerization (MP) there are pores with 1-5 microns in diameter. At 24 hours there are pseudomycelium anode cells in gemmation, while at 48 hours the pseudomycelium formation as well as gemmation is increased.
Conclusions
Acrylic resin elaborated with the ther-mopolimerization injection technique presents a surface with less defects of the three studied techniques and there is no significant difference in the number of adhered cells at 24 and 48 hours after inoculation.
Key words: Candida albicans; scanning electron microscopy; prosthetic stomatitis; adherence
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