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Agrociencia
versión On-line ISSN 2521-9766versión impresa ISSN 1405-3195
Resumen
GARZA-LOPEZ, Paul M.; KONIGSBERG, Mina; SAUCEDO-CASTANEDA, Gerardo y LOERA, Octavio. Differential profiles of Beauveria bassiana (Bals.-Criv.) Vuill. as a response to CO2: production of conidia and amylases. Agrociencia [online]. 2011, vol.45, n.7, pp.761-770. ISSN 2521-9766.
Conidia production of entomopathogenic fungi Beauveria bassiana (Bals.-Criv.) Vuill. is performed in a solid state culture (SSC) using agricultural products and their residues, such as rice (Oryza sativa) and sugarcane (Saccharum ojjicinarum) bagasse, where CO2 can be accumulated as a product of metabolism. In this study rice (Oryza sativa var. japonica) was used as substrate using two atmospheric compositions: normal air and other enriched with CO2 (5 %), through exchanges every 12 or 24 h. The effect of these treatments was determined on the conidial production and amylase enzymes by B. bassiana, using a completely randomized design and analysis of variance using Tukey-Kramer test (p< 0.05). Maximum conidia production per g of initial dry substrate (gssi) was obtained with the normal atmosphere (1.14×109 conidia gssi-1), while with 5 % of CO2 the conidia production decreased up to 85 % (1.8×108 conidia gssi-1). However, in this latter treatment amylase activity increased three times (16.58 UI gssi-1) with respect to the normal atmosphere (5.41 UI gssi-1). These results are important for the conidia and enzymes production by fungi in SSC, with the recommendation to modify the concentration of CO2, as it is required to favor the type of product.
Palabras llave : Beauveria bassiana; Oryza sativa; carbon dioxide; solid medium culture; yield of conidia; enzymatic activity.