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Revista odontológica mexicana
versión impresa ISSN 1870-199X
Resumen
MARTINEZ-CORTES, Mildred; TINAJERO-MORALES, Carlos; ROSALES, Carlos y URIBE-QUEROL, Eileen. Cytotoxicity assessment of three endodontic sealing cements used in periapical surgery. In vitro study. Rev. Odont. Mex [online]. 2017, vol.21, n.1, pp.40-48. ISSN 1870-199X. https://doi.org/10.1016/j.rodmex.2017.02.010.
Introduction:
Presently there are many retrofilling materials in themarket, nevertheless, little is known about their toxicity on gingival fibroblasts.
Objective:
To assess cytotoxicity of three materials tohuman gingival fibroblasts and L929 mouse fibroblasts cell line.
Material and methods:
EndoSequence® BC RRMTM (ERRM; rootrepair material), white MTA Angelus® (MTA) and intermediaterestoration material (IRM®) conditioned media were obtained whenmaterials were freshly mixed, at setting time and after 1, 24 and72 hours of setting time. Cell morphology was assessed with lightmicroscopy and cell viability was assessed through mitochondrialmetabolic activity with 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Statistical analysis was conducted withANOVA.
Results:
We found that ERRM material did not exhibitcytotoxic effects on used fibroblast, nevertheless, MTA and IRM® respectively exhibited moderate and severe cytotoxicity, thusindicating the materials were not fully harmless.
Conclusion:
Bioceramic cements like ERRM could be considered the mostcompatible retrofilling-materials.
Palabras llave : Cell morphology; cytotoxicity; retro-filling; endodontic sealers; MTT; inflammation.
