Servicios Personalizados
Revista
Articulo
Indicadores
- Citado por SciELO
- Accesos
Links relacionados
- Similares en SciELO
Compartir
Revista mexicana de ciencias pecuarias
versión On-line ISSN 2448-6698versión impresa ISSN 2007-1124
Resumen
VAZQUEZ-GARCIDUENAS, Ma. Soledad; MELENDEZ-CEJA, Silvia Cristina y VAZQUEZ-MARRUFO, Gerardo. A multiplex PCR assay for detection of genus Salmonella, subspecies I, and serotype Typhimurium in milk. Rev. mex. de cienc. pecuarias [online]. 2015, vol.6, n.3, pp.329-335. ISSN 2448-6698.
Gastrointestinal diseases caused by foodstuffs contaminated with Salmonella enterica implicate important social and economic issues, making it necessary to have fast, sensitive, and specific systems for opportune detection of the pathogen. An endpoint PCR protocol for identification of S. enterica in milk is presented using primer pairs previously validated as specific to the genus Salmonella (STM3098-f2/STM3098-r2), subspecies enterica (subspecies I) (STM4057-f/STM4057-r), and serotype Typhimurium (STM4497-f/ STM4497-r). This protocol allowed for detection of 1 CFU/25 mL of spiked milk after a 12 h pre-enrichment period by means of simple and multiplex PCR assay with the three used primer pairs. The designed protocol can be applied in sanitary survey programs for detection of S. enterica in milk.
Palabras llave : Salmonella enterica; Milk; Multiplex PCR.