Resumen

LIANG, Yuan; YIN, Yanbo; ZHOU, Haifeng  y  TIAN, Yuanyu. Enhanced Enzymatic Saccharification of Mixed Sawdust Wastes: Comparison of SPORL, Dilute Acid, Formic Acid, and Ethanol Organosolv Pretreatments. J. Mex. Chem. Soc [online]. 2021, vol.65, n.3, pp.297-307.  Epub 14-Feb-2022. ISSN 1870-249X.  https://doi.org/10.29356/jmcs.v65i3.1427.

Utilization of the huge quantity of sawdust wastes is urgent. In this study, SPORL, dilute acid pretreatment (DA), formic acid pretreatment (FA), ethanol pretreatment (EtOH/H2O), and sulfuric acid catalyzed ethanol pretreatment (EtOH/H2O/H2SO4), on improving enzymatic hydrolysis of mixed sawdust wastes were comprehensively compared. EtOH/H2O/H2SO4 was the most effective pretreatment in lignin removal from sawdust fiber cell wall, while FA was much more effective in hemicellulose removal. After the pretreatments, the crystallinity of cellulose increased because of the removal of amorphous hemicellulose and lignin. Moreover, the fiber surface became coarse and porous, especially after EtOH/H2O/H2SO4, the structure was destroyed into fragments, which enhanced the cellulase accessibility of cellulose. Therefore, the glucose yield of EtOH/H2O/H2SO4 pretreated substrate was highest among these five pretreatments, achieved at 91.4% with a cellulase loading of only 10 FPU/g glucan.

Palabras llave : Mixed sawdust wastes; pretreatments; enzymatic hydrolysis; delignification.

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