Resumen

USUGA-MONROY, Cristina; ECHEVERRI-ZULUAGA, José Julián  y  LOPEZ-HERRERA, Albeiro. Molecular and serological detection of bovine leukemia virus in a population of Holstein cows, from Colombia. Rev. mex. de cienc. pecuarias [online]. 2018, vol.9, n.2, pp.387-399. ISSN 2448-6698.  https://doi.org/10.22319/rmcp.v9i2.4232.

Enzootic bovine leukemia (EBL) is a malignant, systemic disease, high morbidity (70 %) and low mortality, caused by infection with the bovine leukemia virus (BLV). EBL mainly affects dairy cattle; the milk production decreases of 2.5 to 5 % from the herd; also cause immunosuppression allowing other pathogens affecting the production and reproduction of animals. The aim was to determine the presence or absence of BLV through two diagnostic methodologies: a serological technique (ELISA) and a molecular technique (PCR). A total of 1,000 blood samples were taken from Holstein cows distributed in 10 municipalities in Antioquia Department. A PCR was performed to obtain a 444 bp PCR product corresponding to a highly conserved region of the viral env gene, this gene encodes the envelope protein gp51. An indirect ELISA was performed against the gp51 protein for these animals. The PCR detected fewer positive samples (474) that ELISA test (546). The sensitivity of PCR was 74.91 % and the specificity was 85.68 %, compared with the ELISA. The degree of agreement between tests was moderate (kappa=0.59). The municipality with the highest molecular prevalence was Rionegro (73.91 %), while the highest seroprevalence went to the municipality of El Retiro (85.19 %). The BLV has increased its presence in the dairy municipalities in the region; the detection of this pathogen will not only allow to determine the prevalence, it will also allow to use the data obtained in programs of prevention and control of the disease.

Palabras llave : Bovine leukemia virus; Diagnosis; ELISA; Nested-PCR.

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