Resumo

SANCHEZ-ALEMAN, Miguel Ángel; ARAIZA, Javier  e  BONIFAZ, Alexandra. Isolation and Characterization of Wild Sporothrix schenkii Strains and Investigation of Sporototrichin Reactors. Gac. Méd. Méx [online]. 2004, vol.140, n.5, pp.507-512. ISSN 2696-1288.

We conducted a study in the southern mountains of the Mexican State of Oaxaca that consisted of isolation of wild Sporothrix schenckii strains obtained from soil samples and investigation of positive reactors to skin test reaction with sprotrichin antigen. The study was conducted by means of recollection of soil samples and processing of these with dilution methods and fungal isolation in ordinary culture media Sabouraud simple Agar with and without antibiotics (SS, SA). Suspected strains underwent dimorfism, melanin formation, and virulence confirmation tests. Investigation of positive reactors to sporotrichin Y (yeast) was also conducted. Three supposed strains were identified due to their reproductive characteristics, melaninproduction, and virulence. In the community; 144 individuals were studied, ofwhom6.25%werepositivetosporotichin. Isolation of virulent strains o/Sporotrhix schenkii from nature (soil) andprimoinfection of a percentage of the studied population were confirmed.

Palavras-chave : Sporothrix schenckii; sporotrichosis; isolation; sporotrichin.