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Revista de investigación clínica
versão On-line ISSN 2564-8896versão impressa ISSN 0034-8376
Resumo
CABRERA-MENDOZA, Brenda et al. Brain Gene Expression-DNA Methylation Correlation in Suicide Completers: Preliminary Results. Rev. invest. clín. [online]. 2020, vol.72, n.5, pp.283-292. Epub 09-Abr-2021. ISSN 2564-8896. https://doi.org/10.24875/ric.19003250.
Background:
Gene expression alterations have been implicated in suicide pathology. However, the study of the regulatory effect of DNA methylation on gene expression in the suicidal brain has been restricted to candidate genes.
Objective:
The objective of the study was to identify genes whose expression levels are correlated with DNA methylation in the prefrontal cortex of suicides.
Methods:
Postmortem prefrontal cortex samples from 21 suicides and six non-suicides were collected. Transcriptomic and DNA methylation profiles were evaluated with microarrays; cis correlations between gene expression and CpG methylation were screened. We then analyzed the presence of transcription factor (TF) binding sites (TFBS) at CpG sites correlated with gene expression. Gene expression of TFs involved in neurodevelopmental binding to predicted TFBS was determined in the BrainSpan database.
Results:
We identified 22 CpG sites whose methylation levels correlated with gene expression in the prefrontal cortex of suicides. Genes annotated to identified CpG sites were involved in neurodevelopment (BBS4, NKX6-2, AXL, CTNND1, and MBP) and polyamine metabolism (polyamine oxidase [PAOX]). Such correlations were not detected in the non-suicide group. Nine TFs (USF1, TBP, SF1, NRF1, RFX1, SP3, PKNOX1, MAZ, and POU3F2) showed differential expression in pre- and post-natal developmental periods, according to BrainSpan database.
Conclusions:
The integration of different omic technologies provided novel candidates for the investigation of genes whose expression is altered in the suicidal brain and their potential regulatory mechanisms. (REV INVEST CLIN. 2020;72(5):283-92)
Palavras-chave : Suicide; DNA methylation; Gene expression; Cerebral cortex; Microarrays; Transcription factors; Neurodevelopment.