Resumo

NAVARRO-MALDONADO, María del Carmen et al. Epitelial cells derived from Ovis canadensis mexicana thawed skin tissue for a germplasm bank. Acta Zool. Mex [online]. 2015, vol.31, n.2, pp.275-282. ISSN 2448-8445.

Several mammal species of ecological interest in our country boast some category of risk or danger of extinction. With the purpose of guarding the genetic information of these species, researchers have developed techniques of ex situ conservation, which are key components of global conservation programs that contribute for the storage of the genome. This storage is carried out through the collection of cells and tissues embedded in germplasm banks, which are the most efficient solution to the critical panorama of the threatened wild fauna. In the present work, for the very first time in Mexico, epithelial (keratinocytes) and connective tissue (fibroblasts) cells were derived in culture on day 28, from Ovis canadensis mexicana after 54 days thawed skin tissue, to create a germplasm bank.

Palavras-chave : germplasm bank; epithelial cells; bighorn sheep.