Serviços Personalizados
Journal
Artigo
Indicadores
- Citado por SciELO
- Acessos
Links relacionados
- Similares em SciELO
Compartilhar
Revista mexicana de fitopatología
versão On-line ISSN 2007-8080versão impressa ISSN 0185-3309
Resumo
HERNANDEZ-DE LA CRUZ, Miguel et al. Serological and molecular detection of virus PVYN and its variant PVYNTN in potato (Solanum tuberosum L.) and alternate hosts in Tapalpa, Mexico. Rev. mex. fitopatol [online]. 2007, vol.25, n.2, pp.167-172. ISSN 2007-8080.
Sampling for the presence of PVYN virus and its variant PVYNTN, as well as for weeds and insects associated with cultivation of potato (Solanum tuberosum), was carried out in fields intended for seed production in Tapalpa, State of Jalisco, Mexico. Forty one cultivars were analyzed, 19 from Tapalpa and 22 from Toluca, State of Mexico, by serological and molecular techniques. DAS-ELISA immunoassays revealed that cv. Malinche from Tapalpa was positive to PVYN, whereas a RT-PCR-based analysis identified the PVYNTN variant in Marinca from Toluca, as well as in Gigant, Malinche, and Alpha from Tapalpa. Among the different weed species associated to potato crop, Solanum nigrescens locally known as hierba mora, was found to be a natural reservoir for PVYN. The aphid Myzus persicae was identified to carry PVYNTN variant and it is considered as a probable vector. A 594 bp fragment, derived from samples that resulted positive for PVYNTN was amplified by RT-PCR, cloned and sequenced with 98% homology to an isolate from Canada, according to the sequence reported in the NCBI. These results confirmed the presence of the PVYNTN variant in commercial potato fields in Tapalpa, Jalisco, Mexico.
Palavras-chave : Myzus persicae; Solanum nigrescens; Solanum nigrum; DAS-ELISA; RT-PCR.