Resumo

FLORES CORDOVA, Maria Antonia et al. Reduction in the in vitro Germination of Conidia of Alternaría alternata Isolated from Eruca sativa with Juice of Broccoli. Rev. mex. fitopatol [online]. 2013, vol.31, n.2, pp.180-190. ISSN 2007-8080.

The fungus Alternaría alternata, is considered one of the major plant pathogens worldwide causing the symptom of leaf spot in vegetables. Glucosinolates are natural products derived from broccoli, containing nitrogen and sulfur, with antifungal activity. Based on the above, the present study aimed to evaluate the effectiveness of broccoli juice in vitro on germination of conidia of A. alternata. To carry out the experiment, leaves of Eruca satíva (arugula) with symptoms of leaf spot were collected, from which the fungus A. alternata was isolated and identified. Conidia of the fungus were placed on PDA medium with different concentrations of glucosinolates found in broccoli juice 0.15, 0.11, 0.07, 0.04, 0.01, and 0 µg/µL. It was observed that the concentrations 0.07, 0.1, 0.15 µg/µL inhibited 100 % spore germination of A. alternata at 6 h evaluation. The average lethal concentration LC₅₀ was 0.028 µg/µL. In conclusion, it can be mentioned that the glucosinolates present in broccoli juice, have an antifungal potential and can be used as post-harvest treatment, in order to reduce the damage caused by A. alternata in vegetables.

Palavras-chave : Arugula; glucosinolates; antifungal; molecular identification; inhibition.

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