Resumo

GONZALEZ-GARZA, Ramiro. Evolution of diagnostic technics for plant viruses. Rev. mex. fitopatol [online]. 2017, vol.35, n.3, pp.591-610. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1706-1.

The symptoms caused by viruses in diseased plants were the first way to detect and identify the viruses that affected them and named them according to the symptoms they produced. The use of differential plants infected by mechanical transmission, by grafting or vectors, increased the ability to detect and identify many of the phytopathogenic viruses and also led to confounding other diseases caused by other infectious agents with viruses. Serology detection uses the interaction of the viral protein as an antigen against the antibodies produced against them by a vertebrate. The first serological methods used for viral detection were antigen-antibody precipitation in liquid medium, followed by agar double diffusion and finally by enzyme-linked immunosorbent assay, very economical in the use of reagents, very sensitive and uses a Solid medium for antigen or antibody immobilization which may be different plastics or membranes. Detection of viral nucleic acid by nucleic acid hybridization or polymerase chain reaction is more sensitive than serology, and the latter method combined with second generation sequencers (Next Generation Sequencing) have revolutionized detection of viruses in plants.

Palavras-chave : Detection of viruses; differential plants; serology; ELISA; nucleic acid hybridization; PCR; NGS.

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