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Universidad y ciencia
versão impressa ISSN 0186-2979
Resumo
GARCIA-RODRIGUEZ, MM; GALLEGOS-MORALES, I; VARGAS-MANCILLA, J e DIAZ-ZAGOYA, JC. Comparative evaluation of the cryoprotective effect of linoleic and oleic acids on bovine oocytes. Universidad y ciencia [online]. 2011, vol.27, n.1, pp.103-107. ISSN 0186-2979.
The cryoprotective effect of linoleic and oleic acids on bovine oocytes, comparatively with glycerol, was studied using stereoscopic and electronic microscopy. The low temperature (-196°C) damaged all the oocytes treated with oleic acid, 25% of those treated with linoleic acid and 15% when they were protected with glycerol. The damage was not detected adequately by stereoscopic microscopy, however it was clearly defined using electronic microscopy.
Palavras-chave : Oocytes; cryopreservation; linoleic acid; oleic acid; glycerol.