Resumo

OSORIO SAENZ, Antelmo et al. Cryopreservation of chrysanthemum shoot-tips (Dendranthema grandiflorum Kitam) by encapsulation-dehydration and vitrification. Rev. Chapingo Ser.Hortic [online]. 2011, vol.17, n.spe2, pp.33-43. ISSN 2007-4034.

The development of biotechnological methods of cryopreservation allows safe, long-term germplasm storage of valuable species such as chrysanthemum, which is one of the most important ornamental plants in Mexico and around the world. This study used the cryogenic methods of encapsulation-dehydration and vitrification to conserve chrysanthemum cv. Indianapolis White plants grown in vitro. Results showed similar behavior with both procedures during tissue recovery. The highest survival rates after cryopreservation were 60 % for encapsulation-dehydration and 74 % with vitrification. However, regeneration in both cases passed through a primary phase of callus formation and rates of obtention of new plants were low, 12 and 10 %, respectively. Therefore, further research is needed to improve cryoprotectective conditions in order to increase rate of recovery of chrysantemum plants.

Palavras-chave : Biotechnology; tissue culture; germplasm; Indianapolis White; liquid nitrogen.

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