Resumo

PEQUENO-GRANADO, I.L. et al. Organogenic induction of Jatropha curcas L. from young leaves. Polibotánica [online]. 2015, n.39, pp.79-89. ISSN 1405-2768.

Jatropha curcas L., known in Mexico as "piñoncillo", is an oil producing species, which has received much attention in recent years as a source for biodiesel production because their seed yield from 46 to 64% oil. However, Jatropha production has shown limited success due to heterogeneous seed yield and oil content. For this reason, the selection of genotypes with superior performance characteristics to be propagated on a large scale has increased. Therefore, clonal propagation by plant tissue technique offers an alternative for genetic omogeneity. So, in this research, the organogenic induction from young leaves of a Mexican Jatroppha genotype was studied. The establishment of in vitro asceptic explants in Murashige & Skoog 1962 (MS) culture media was accomplished after 14 days with 70% asceptic explants. With respect to the induction of buds, greater response was achieved in treatment one, using the same basic media supplemented with 1.0 mg L^-1 6-benzyl-aminopurine (BA) and 0.5 mg L^-1 indole-3-butyric acid (IBA), achieving bud formation after 60 days, viability was 37.5%, while the number of adventitious buds was 2.2, all treatments showed callus association. In bud proliferation, the greatest response was in treatment two, with the addition of 0.5 mg L^-1 BA, with an average of 5.14 shoots per explants after four weeks.

Palavras-chave : leaf; in vitro; buds; shoots; Jatropha curcas L.; induction.