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Agrociencia
versão On-line ISSN 2521-9766versão impressa ISSN 1405-3195
Agrociencia vol.41 no.3 Texcoco Abr./Mai. 2007
Plant protection
Isolation and identification of Physalis philadelphica LAM. volatiles
1Universidad Politécnica de Francisco I. Madero. 42660. Tepatepec, Hidalgo. (hcalyecac@yahoo. com).
2Entomología y Acarología, Universidad Politécnica de Francisco I. Madero. 42660. Tepatepec, Hidalgo.
3Botánica, Campus Montecillo Colegio de Postgraduados. 56230. Montecillo, Texcoco, Estado de México.
4Centro Universitario UAEM Tenancingo. Universidad Autónoma del Estado de México. Ex Hacieda de Santa Ana. 52400. Carretera Tenancingo-Villa Guerrero. Tenancingo, Estado de México.
This study was conducted to isolate and identify the volatile components of husk tomato Physalis philadelphica Lam. Volatiles were collected by washing with hexane and through dynamic aeration (DA) and solid phase micro-extraction (SPME). The volatiles collected were isolated, analyzed and identified with gas chromatography (GC) coupled with mass spectrometry (GC-MS). The final compounds collected by DA and SPME were identified as methyl salicylate, β-caryophyllene, β-elemene, decanal and germacrene D. The compounds collected by hexane washing were β-caryophyllene, ethyl linolenate, methyl salicylate, β-pinene, hexadecanoic acid, methyl linolenate, benzyl alcohol, D germacrene, 3 carene, β-elemene and decanal.
Key words: Dynamic aeration; allelochemicals; GC; GC-MS; solid phase micro-extraction; husk tomato
El objetivo de este trabajo fue aislar e identificar los componentes volátiles del tomate de cáscara Physalis philadelphica Lam. Por lavado con hexano, aireación dinámica (AD) y micro extracción en fase sólida (MEFS) se aislaron volátiles de P. philadelphica, los cuales se analizaron e identificaron por cromatografía de gases (CG) y CG acoplada a espectrometría de masas (CG-EM). Los compuestos recolectados por AD y MEFS se identificaron como salicilato de metilo, β-cariofileno, β-pineno, alcohol bencílico, 3-careno, β-elemeno, decanal y germacreno D. Los componentes recolectados lavando las plantas con hexano fueron β-cariofileno, linolenato de etilo, salicilato de metilo, β-pineno, ácido hexadecanoico, linolenato de metilo, alcohol bencílico, germacreno D, 3-careno, β-elemeno y decanal.
Palabras clave: Aireación dinámica; aleloquímicos; CG; CG-EM; micro extracción en fase sólida; tomate de cáscara
LITERATURA CITADA
Angelopoulos, N. G., A. M. Hooper, S. P. Maniar, J. A. Pickett, and L. J. Wadhams. 1999. A novel approach for isolation of volatile chemicals released by individual leaves of a plant in situ. J. Chem. Ecol. 25: 1411-1425. [ Links ]
Bartle, K. D. 1993. Introduction to the theory of chromatographic separations with reference to gas chromatography. In: Gas Chromatography. A Practical Approach. Baugh, P. J. (ed). Oxford University Press Inc. New York. pp: 1-13. [ Links ]
Baur, P., H. Marzouk, J. Schönherr, and H. Bauer. 1996. Mobilities of organic compounds in plant cuticles as affected by structure and molar volumes of chemicals and plant species. Planta 199: 404-412. [ Links ]
Bautista, M., N., y O. Morales G. 2000. Insectos defoliadores y barrenadores de mayor importancia en hortalizas. In: Temas Selectos en Fitosanidad y Producción de Hortalizas. Bautista M., N., A. D. Suárez V., y O. Morales G. (eds). Instituto de Fitosanidad, Colegio de Postgraduados. Montecillo, México. pp: 1-2. [ Links ]
Bernays, E. A., and R. F. Chapman. 1994. Host-plant selection by phytophagous insects. Chapman & Hall, New York. 312 p. [ Links ]
Bock, M. A., J. Sánchez P., L. J. McKee, and M. Ortiz. 1995. Selected nutritional and quality analyses of tomatillos (Physalis ixocarpa). Plant Foods Hum. Nutr. 48: 127-133. [ Links ]
Brazzel, J. R., L. D. Newsome, T. S. Rousel, C. Lincoln, F. J. Williams, and G. Barnes. 1953. Bollworm and tobacco budworm as cotton pest in Louisiana and Arkansas. La. Agric. Exp. Stn. Tech. Bull. 482: 47. [ Links ]
Bukasov, S. M. 1963. Las Plantas Cultivadas en México, Guatemala y Colombia. Lima. IICA. Publicación Especial 20. 240 p. [ Links ]
Buttery, R. G., L. C. Ling, and D. M. Light. 1987. Tomato leaf volatile aroma components. J. Agric. Food Chem. 35: 1039-1042. [ Links ]
Calyecac-Cortero, H. G., J. Cibrián-Tovar, J. López-Collado y R. García-Velasco. 2006. Emisores de los volátiles de atracción de Trichobaris championi Barber. Agrociencia 40: 655-663. [ Links ]
Dicke, M., T. A. Van Beek, M. A. Posthumus, N. Ben Dom, H. Van Bokhoven, and A. E. De Groot. 1990. Isolation and identification of volatile kairomone that affects acarine predatorprey interactions. Involvement of host plant in its production. J. Chem. Ecol. 16: 381-396. [ Links ]
Dickens, J. C. 2000. Sexual maturation and temporal variation of neural responses in adult Colorado potato beetles to volatiles emitted by potato plants. J. Chem. Ecol. 26: 1265-1279. [ Links ]
Elmaci, Y., and T. Altug. 2002. Flavour evaluation of three black mulberry (Morus nigra) cultivars using GC/MS, chemical and sensory data. J. Agric. Food Agric. 82: 632-635. [ Links ]
Fraser, A. M., W. L. Mechaber, and J. G. Hildebrand. 2003. Electroantennographic and behavioral responses of the sphinx moth Manduca sexta to host plant headspace volatiles. J. Chem. Ecol. 29: 1813-1833. [ Links ]
Fuentes Contreras, E., E. Gianoli, E. Quiroz, C. C. Ramírez y H. M. Niemeyer. 2001. Ecología de las relaciones entre áfidos y plantas. In: Anaya, A. L., F. Espinosa García, y R. Cruz-Ortega (coord). Relaciones Químicas entre Organismos: Aspectos Básicos y Perspectivas de su Aplicación. Instituto de Ecología, Plaza y Valdez Editores. México. pp: 305-375. [ Links ]
Gu, C, S. Lu, and L. Gu. 1999. Abstraction of Valeriana pseudofficinalis flower essential oil and analysis by GC/MS. Chem. Ind. For. Prod. 19: 75-78. [ Links ]
Hernández, R. F., y J. L. Carrillo S. 1973. Parasitismo natural de Trichogramma spp. sobre el gusano del fruto de Physalis sp. en el estado de Morelos. Agric. Téc. Méx. 3(7): 255-258. [ Links ]
Loughrin, J. H., T. R. Hamilon-Kemp, H. R. Burton, R. A. Andersen, and D. F. Hildebrand. 1992. Glycosidically bound volatile components of Nicotiana silvestris and N. suaveolens flowers. Phytochemistry 31: 1537-1540. [ Links ]
Millar, J. G., and K. F. Haynes. 1998. Methods in Chemical Ecology: Chemical Methods. Vol. 1. Kluwer Academic Publisher. 389 p. [ Links ]
Morton, J.F. 1987. Mexican husk tomato. In: Fruits of Warm Climates. Morton, J. F. (ed). Creative Resource Systems, Inc. Miami Florida. pp: 434-437. [ Links ]
Mozuraitis, R., M. Stranden, M. I. Ramirez, A. K. Borg-Karlson, and H. Mustaparta. 2002. Germacrene D increases attraction and oviposition by the tobacco budworm moth Heliothis virescens. Chem. Senses 27: 505-509. [ Links ]
Pawliszyn, J. 1997. Solid Phase Microextraction. Theory and Practice. Wiley-VCH. New York. 247 p. [ Links ]
Peña L., A., y F. Márquez S. 1990. Mejoramiento genético de tomate de cáscara (Physalis ixocarpa Brot.). Rev. Chapingo. Año XV, 71-72: 84-88. [ Links ]
Penton, Z. E. 1996. Sample preparation for gas chromatography with solid-phase extraction and solid-phase microextraction. Ad. Chromat. 37: 205-236. [ Links ]
Pérez M., L., y J. Granados A. 2001. Fertilización nitro-fosfórica en tomate de cáscara (Physalis ixocarpa Brot.) de riego en Irapuato, Guanajuato, México. Acta Universitaria 11: 19-25. [ Links ]
Pino, J. A., P. Fernandes, G. Freitas, and R. Marbot. 2003. Chemical composition of the leaf oil of Juniperus cedros Webb. et Berth. From Madeira. J. Essential Oil Res. 15: 331-332. [ Links ]
Rao, P. V. S., J. P. Geiger, J. Einhorn, C. Alosse, B. Rio, M. Nicole, S. Savry, and A. Ravise. 1988. Isolation of methyl linolenate, a new antifungal compound from Arachis hypogaea L. leaves infected with Puccinia arachidis Speg. Olégineux 43: 173-176. [ Links ]
Robards, K., P. R. Haddad, and P. E. Jackson. 1994. Principles and Practice of Modern Chromatographic Methods. Academic Press. New York. 231 p. [ Links ]
Schulz, H., H. Krüger, N. Herchert, and E. R. J. Keller. 2000. Occurrence of volatile secondary metabolites in selected Allium wild types. J. Appl. Bot. 74: 119-121. [ Links ]
SIACON (Sistema de Información Agropecuaria y de Consulta). 2005. Información de la Producción Agrícola Nacional. Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA). Disponible en línea en Disponible en línea en http://www.siea.sagarpa.gob.mx/sistemas/siacon/SIACON.html . Consultado el 27 de agosto de 2006. [ Links ]
Smith, P. M. 1976. The Chemotaxonomy of Plants. Elsevier, New York. 313 p. [ Links ]
Stein, S., A. Levitsky, O. Fateev, D. Tchekhovskoi, and G. Mallard. 2000. NIST 98. Mass Spectral Search Program. NIST/EPA/NIH Mass Spectral Library. USA. [ Links ]
Stevens, I. D. R. 1998. Chemical aspects of pheromones, isolation and structure determination. In: Insect Pheromones and their Use in Pest Management. Howse, P. E., I. D. R. Stevens, and O. T. Jones (eds). Chapman and Hall, London. pp: 180-225. [ Links ]
Tammela, P., M. Nygren, I. Laako, A. Hopia, H. Vuorela, and R. Hiltunen. 2003. Volatile compound analysis of ageing Pinus sylvestris L. (Scots pine) seeds. Flavour Frag. J. 18: 290-295. [ Links ]
Thaler, J. S., M. A. Farag, P. W. Paré, and M. Dicke. 2002. Jasmonate-deficient plants have reduced direct and indirect defense against herbivores. Ecol. Letters 5: 764-774. [ Links ]
Tingle, F. C., E. R. Mitchell, and R. R. Heath. 1990. Preferences of mated Heliothis virescens and H. subflexa females for host and nonhost volatiles in a flight tunnel. J. Chem. Ecol. 16: 2889-2898. [ Links ]
Weurman, C. 1969. Isolation and concentration of volatiles in food odor research. J. Agr. Food Chem. 17: 370-384. [ Links ]
Zygadlo, J. A., and N. R. Grosso. 1997. Volatile constituents from the flowers of Solanum stuckertii and S. incisum. J. Essential Oil Res. 9: 111-113. [ Links ]
Received: January 2006; Accepted: November 2006