Resumo

VAZQUEZ R., María Luisa; HERNANDEZ L., Alejandra  e  RETCHKIMAN C., Berta. Development and validation of an analytical method by high performance liquid chromatography for determination of indomethacin in cream. Rev. mex. cienc. farm [online]. 2011, vol.42, n.1, pp.45-51. ISSN 1870-0195.

An easy, quick and completely automatizated analytical method was developed by High Performance Liquid Chromatography (HPLC) with UV detection for quantitative determination of Indomethacin in 2.5% cream. Different conditions for its determination were tested, finding the best conditions using a L column₁ (Supelcosil LC18, 250 mm x 4.6 mm, 5 μm), a movil phase with metanol and phosphate buffer pH 7.1 (70:30), flow speed 1.0 mL/min and detection lambda of 254 nm. The method is quick and linear from 10 to 50 μg/mL ((r² = 0.999), accurate (IC 100.32±4.5%) and adequate precision (CV< 1.5%), and it results in an adequate analytical method to quantification of Indomethacin in a quality control test.

Palavras-chave : indomethacin; HPLC; pharmaceuticals.