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Revista odontológica mexicana
versão impressa ISSN 1870-199X
Rev. Odont. Mex vol.10 no.2 Ciudad de México Jun. 2006
https://doi.org/10.22201/fo.1870199xp.2006.10.2.15905
Trabajos originales
Efecto del nimesulide en la formación de osteoclastos inducidos por estrés mecánico
Effect of the nimesulide in osteoclastic formation induced by mechanic stress
* Facultad de Estudios Superiores Iztacala. Universidad Nacional Autónoma de México. México.
En este estudio evaluamos el efecto del nimesulide, un inhibidor débil de la biosíntesis de prostaglandinas con el objetivo de observar su efecto en la formación de osteoclastos. A 60 ratas Wistar machos de 250 ± 25 g de peso se les colocó un resorte en forma de U de níquel-titanio de 0.012 pulgadas, entre los primeros molares maxilares, Los animales se dividieron en cuatro grupos, a tres de éstos se les administró nimesulide a dosis baja, media y alta. Fueron sacrificadas a las 24, 48 y 72 horas, se les disecó el maxilar que se descalcificó con EDTA, para incluirse en paraplast y obtener cortes de 7 micrómetros que se tiñeron con hematoxilina y eosina. Resultados: El nimesulide disminuyó la población de osteoclastos en todas las dosis. A las 24 horas la inhibición fue dependiente de la dosis. A las 48 y 72 horas todas las dosis fueron efectivas. En el grupo testigo, el número de osteoclastos se incrementó gradualmente a las 24, 48 y 72 horas.
Palabras clave: Nimesulide; osteoclastos; estrés mecánico
In the present study we observed the effect of nimesulide with the objective of evaluating their effect in osteoclast formation. We used Sixty male Wistar rats weighing around 250 ± 25 g. A uniform standardized U spring, made of 0.012-inch nickel-titanium wire was set in each animals mouth between the right and left upper first molar. The animals were divided into 4 groups and three of them were prescribed with low, medium and high doses of nimesulid. The animals were killed at 24, 48 and 72 hours after the placement of the appliances. The maxillae was removed and fixed in formaldehyde for 48 hours, and subsequently decalcified in EDTA (pH 7.4), and included in Paraplast. Serial sections of 7 micrometers were cut and stained with hematoxylin and eosin. Results: the NSAIDs nimesulide in all doses inhibited the formation of osteoclasts. At 24 h the osteoclast inhibition was dosis dependent. At 48 and 72 h all doses were effective. In control group, osteoclasts increase gradually at 24, 48 and 72 h.
Key words: Nimesulid; osteoclast; mechanical stress
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