Resumo

BASAVAIAH, Kanakapura et al. Titrimetric and Spectrophotometric Assay of Pantoprazole in Pharmaceuticals Using Permanganate. J. Mex. Chem. Soc [online]. 2009, vol.53, n.1, pp.34-40. ISSN 1870-249X.

Abstract Titrimetric and spectrophotometric assay of pantoprazole sodium (PPS) using permanganate as the oxidimetric reagent is described. In titrimetry, PPS is treated with a measured excess of permanganate in H₂SO₄ medium followed by the determination of unreacted oxidant by back titrating with a standard iron(II) solution. Spectrophotometry involves addition of a known excess of permanganate to PPS in H₂SO₄ medium followed by the measurement of absorbance of the residual permanganate at 545 nm. In both the methods, the amount of permanganate reacted corresponds to the PPS content. Experimental conditions that provide wide linear range, maximum sensitivity and selectivity, and accuracy and precisions have been optimized. In titrimetry, the calculations are based on a 1:1 (PPS : KMnO₄) reaction stoichiometry and the method is applicable over 1.0-7.0 mg range. In spectrophotometry, Beer's law is obeyed over the concentration range 15.0-150.0 µg mL^-1. The linear regression equation of the calibration graph is A = 0.78 + 0.005 C with a regression coefficient (r) of 0.9982 (n = 11). The apparent molar absorptivity is calculated to be 2.213 × 10³ l mol^-1cm^-1 and the Sandell sensitivity is 0.1954 µg cm^-2. The limits of detection (LOD) and quantification (LOQ) calculated as per the ICH guidelines are 0.73 and 2.21 µg mL^-1, respectively. Accuracy and precision of the assays were determined by computing the intra-day and inter-day variations at three different levels of PPS; the intra-day and inter-day RSD was < 3.09 % and the accuracy was better than 3.5 %. The methods were successfully applied to the determination of PPS in three different brands of tablets with good accuracy and precision, and without detectable interference by excipients. The accuracy was further ascertained by placebo blank and synthetic mixture analyses and also by recovery experiments via standard-addition procedure.

Palavras-chave : Pantoprazole; assay; titrimetry; spectrophotometry; permanganate; pharmaceuticals.