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Revista mexicana de ciencias pecuarias
versão On-line ISSN 2448-6698versão impressa ISSN 2007-1124
Resumo
GOMEZ-GOMEZ, Sergio Daniel et al. Detection of porcine reproductive and respiratory syndrome in porcine herds of Baja California, Mexico. Rev. mex. de cienc. pecuarias [online]. 2021, vol.12, n.4, pp.1317-1325. Epub 06-Jun-2022. ISSN 2448-6698. https://doi.org/10.22319/rmcp.v12i4.5778.
The objective of this study was to assess the presence of genotype 2 porcine reproductive and respiratory syndrome virus (PRRSV-2) in Baja California (Baja), as well as the standardization of the qRT-PCR technique. A cross-sectional study was conducted from 2016 to 2017 in farms from Baja. It was obtained 97 blood samples from clinically healthy, not-vaccinated boars and sows. Primers were designed and standardize, in order to perform qRT-PCR tests from the buffy coat. Every positive results were confirmed by sequence studies. It was found that 9.3 % of the samples were positive. The positive samples came from 66.6 % of the sampled regions. This study demonstrates the presence of PRRSV-2 in Baja, therefore, it is necessary to conduct epidemiological studies in order to identify the magnitude of the problem and to establish preventive and control measures.
Palavras-chave : Detection; Mexico; PCR; PRRS; Swine.