Resumo

CASTANEDA-RUELAS, G. M. et al. Adhesion capacity and invasion index of L. monocytogenes strains isolated from food and clinical cases in Mexico. Revista bio ciencias [online]. 2019, vol.6, n.spe, e456.  Epub 14-Nov-2019. ISSN 2007-3380.  https://doi.org/10.15741/revbio.06.nesp.e456.

To evaluate the adherence and invasion capacity of different Listeria monocytogenes genotypes recovered from food samples and clinical cases in Mexico, and to know the pathogenic relationship between both groups of strains. Thirty strains of L. monocytogenes isolated from food (n=23) and clinical cases (n=7) previously typed by serology and pulsed-field gel electrophoresis were evaluated. The virulence phenotype was determined by the adhesion and invasion capacity in HEp-2, HT-29 and Caco2 cells. The L. monocytogenes strains analyzed showed diffuse adherence pattern and cell damage, a marked invasion index associated to the strain (p=0.000), serotype (p=0.018) and genotype (p=0.001), but not regarding to origin (p=0.685) were observed. The 23 strains isolated from food were classified with high (4.3 %), moderate (87.0 %) and low (8.7 %) invasion phenotype according to the phenotype presented by clinical and control strains. The characteristics identified in both strain groups infer that L. monocytogenes independent of its origin is a pathogen with different virulence degree, and supports the foods participation in the bacteria transmission and listeriosis cases in Mexico.

Palavras-chave : Food; Listeria monocytogenes; Listeriosis; Virulence.

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