Resumo

BAEZ-PARRA, K. M. et al. In Vitro Antimicrobial Activity of Methanolic and Hexanic Extracts of Turnera diffusa Against Common Urinary Pathogens. Revista bio ciencias [online]. 2019, vol.6, n.spe, e670.  Epub 03-Set-2019. ISSN 2007-3380.  https://doi.org/10.15741/revbio.06.nesp.e670.

Turnera diffusa Willd. ex Schult. is well known in traditional medicine as aphrodisiac, diuretic, hypoglycemic, tranquilizer and urinary tract antiseptic, here the main focus was the antiseptic effect. The goal of this study was to evaluate the antimicrobial activity of methanolic and hexanic extracts of two varieties of Turnera diffusa Willd. ex Schult against main tract urinary pathogens. Also, the chromatographic profile of the extracts was generated. For the methanolic extract the mobile phase was composed of ethyl acetate, acetic and formic acids and water (100:11:11:27). On the other hand, hexane and acetic acid (7.2:2.9) were used for the hexanic extract. The antimicrobial activity and minimum inhibition concentration were evaluated by the disc diffusion method and microdilution method, respectively, against Klebsiella pneumoniae, Enterococcus faeccalis, Staphylococcus aureus, Escherichia coli and Candida albicans. The methanolic extract revealed the presence of bands from yellow to orange while the hexanic extract showed bands from blue to violet. The results showed antibacterial and antifungal activities of both methanolic and hexanic extracts of Turnera diffusa var. diffusa and Turnera diffusa var. aphrodisiaca against urinary tract pathogenic species, confirming one of their potential medicinal uses.

Palavras-chave : Antimicrobial activity; HPTLC (High performance thin layer chromatography); minimum inhibitory concentration (MIC); Turnera diffusa; urinary tract infections.