Resumo

CARDONA-LOPEZ, M. A. et al. Identification of Escherichia coli pathotypes in ground beef from butcher shops of Guadalajara, Jalisco, Mexico. Revista bio ciencias [online]. 2020, vol.7, e924.  Epub 28-Abr-2021. ISSN 2007-3380.  https://doi.org/10.15741/revbio.07.e924.

According to Mexican Meat Council, in 2017, the national production of beef was 1,915 million tons. Ground bovine meat has been implicated in numerous disease outbreaks since 1982, particularly caused by Escherichia coli pathotypes. From March to November 2014, 100 samples of ground beef, obtained from butcher’s shops in Guadalajara, Jalisco, were analyzed with the purpose of isolating and identifying Escherichia coli pathotypes. To this end, the method proposed by the Bacteriological Analytical Manual (BAM) of the Food and Drug Administration (FDA) was used. Pathotypes were identified by means of the amplification of genes encoding virulence factors, using multiplex PCR. Seventy-six suspect strains for pathogenic Escherichia coli were isolated, from 15 samples. Only 72.36 % (55/76) of the presumptive strains of Escherichia coli amplified the gadA/B gene, confirming the genus and species. Only 7.27 % (4/55) showed the aggR gene encoding for EAEC and 41.18 % (23/55) the elt gene encodes for ETEC’s heat-labile enterotoxin. The potential risk resulting from the consumption of ground beef from butcher’s shops in Guadalajara, Jalisco, was evidenced.

Palavras-chave : Raw ground beef; Butcher’s shop; Escherichia coli pathotype; multiplex PCR.

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