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Revista Chapingo serie ciencias forestales y del ambiente
versão On-line ISSN 2007-4018versão impressa ISSN 2007-3828
Resumo
MEDRANO-HERNANDEZ, Jessica M.; RODRIGUEZ DE LA O, José L.; REYES-TREJO, Benito e PENA-ORTEGA, M. Gisela. Molecular characterization using ISSR primers of Magnolia mexicana DC. from two regions in Zongolica, Veracruz, Mexico. Rev. Chapingo ser. cienc. for. ambient [online]. 2017, vol.23, n.3, pp.427-436. ISSN 2007-4018. https://doi.org/10.5154/r.rchscfa.2017.03.019.
Introduction:
Magnolia mexicana DC. is a threatened species according to NOM-059-SEMARNAT-2010, a situation attributed to the fragmentation and destruction of its habitat. There are no studies on the genetic diversity of M. mexicana, even though it is endemic to our country.
Objective:
To evaluate genetic variability in two M. mexicana populations using ISSR molecular markers.
Materials and methods:
The collections come from Amatitla and Zapotla in Zongolica, Veracruz. The DNA was extracted from the young leaves. A total of 55 ISSR primers were tested; the 10 that produced the highest number of polymorphic bands were selected and amplified by PCR.
Results and discussion:
The ISSR primers showed 86 % polymorphism. Cluster analysis, using Ward's minimum variance method, was able to separate the collections by their geographical origin. Analysis of molecular variance showed that the greatest variability (90.88 %) is found within each population. The Shannon-Weaver diversity index was 0.47 and 0.41 for Amatitla and Zapotla, respectively.
Conclusion:
The M. mexicana populations have not undergone changes in their genetic structure; there is no evidence at genetic level of alterations caused by population reduction or habitat fragmentation.
Palavras-chave : Threatened species; fragmented habitat; genetic variability; Shannon-Weaver index.