INTRODUCTION
Human impact is the main reason for changes in ecosystem services (MEA 2005). Hazardous compounds accumulated in soil affect soil quality. Soil pollution is studied using plant and animal test-systems. Humans also can serve as bioindicators in biomonitoring studies (Kamal and Malik 2017). In vitro experiments with blood samples are a promising approach to detect eco-health. As a body transport medium, blood reflects immediate exposure to changes in the environment (Garg 2017). For example, Furuyama et al. (2009)) showed that ultrafine (20 nm) and fine (200 nm) particles are translocated from alveoli to the extrapulmonary organs via blood circulation and alveolar macrophages.
Soil can influence human health in different ways (Steffan et al. 2018). In vitro tests are able to detect many negative effects of pollutants. The WST-1 assay was successfully applied in biomonitoring studies using animal and plant test objects (Catala et al. 2009, Barriga-Vallejo et al. 2016). The assay principle is based upon the reduction of tetrazolium salts to formazan by dehydrogenases, leading to a colored complex whose quantity can be measured spectrophotometrically and is proportional to the number of viable cells. The reduction of the 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1) reagent are inside and outside the mitochondria and its solubility in the growth medium. This enzymatic activity is performed only by living cells according their metabolic activity (López et al. 2020). Exposure to toxic substances has a negative impact on the body metabolism. Tetrazolium salts are proved to be useful tools for measuring the metabolic activity of cells (Berridge et al. 2005, López et al. 2020). Previously we demonstrated the effectiveness of the WST-1 test to assess salt stress using whole blood samples from Natrix tessellata, Laurenti, 1768 (Koleva et al. 2017). Here we show that WST-1 can be used for evaluation of the effect of water soil extracts on enzyme activity in human whole-blood samples.
MATERIAL AND METHODS
The study areas are the Shumen Plateau Nature Park (NP) and the City Park in Shumen (SCP). We used the same soil samples than in our previous study (Koynova et al. 2017, Koleva et al. 2018). After preparing the soil for laboratory tests, unused soil was stored at -20 ºС. Before the tests, the soil was thawed. To avoid influence of mutagenic metabolic products of soil microorganisms, soil samples were sterilized by autoclaving. Samples of soil suspensions were prepared from one part soil and two parts culture media (Dulbecco’s Modified Eagle Medium with gentamicin and penicillin). They were allowed to stand at room temperature for 24 h to prepare a soil extract of water-soluble compounds. The samples were centrifuged at 1000 g for 10 min. The soil extracts were filtered using a 0.22 μm filter. Ten percent fetal bovine serum was added to each extract.
The blood samples were provided by a young healthy donor. Each treatment sample contained 50 μL whole blood, 450 μL soil extracts, and 50 μL of WST-1 (Roche Applied Science). The control contained 50 μL whole blood, 450 μL complete culture media and 50 μL of WST-1. The samples were gently suspended and incubated for 4 h at 37 ± 1 ºС in 5 % CO2 humidified incubator (Memmert, GmbH).
After treatment, 50 mL of the surface layer of each sample were carefully taken and placed in microplate well. The formazan quantity was measured spectrophotometrically at 450 nm using an enzyme-linked immunosorbent assay (ELISA) reader. The change in enzyme activity was calculated as a percentage of the absorbance of the sample from the absorbance of the control. The test was carried out in three replicates. Student’s t-test was performed. The level of statistical significance was set at P ≤ 0.05.
RESULTS AND DISCUSSION
The Shumen Plateau Nature Park and Shumen city park (Bulgaria) are important green spaces for outdoor recreation (Koynova 2018). Soil quality is a key factor for ecosystem services. Exposure to contaminated soil poses a significant risk to human health (Kamal 2017). So, biomonitoring of soil pollution is vitally important.
In the present study, the influence of water soil extracts on enzyme activity in whole blood samples was evaluated. We observed significant differences between negative control and treated whole-blood samples (Fig. 1). The most notable was negative control samples from the peri-urban Nature Park (SNP). The ability of enzymes in whole-blood samples to reduce tetrazolium salt decreased from 2.9 to 6.6-fold in comparison with the control (P ≤ 0.05). Strong inhibition was measured also after treatment with the sample from the city park (SCP): 3.1-fold in comparison with the negative control (P ≤ 0.05). This data indicates that soil water extracts affected normal metabolic activity. Other studies also reported the influence of toxic pollutants on enzymes (Christensen еt al. 1982, Zolkipli-Cunningham and Falk 2017).
The present study shows that the WST-1 test can be used as a simple and useful tool to assess the presence of water-soluble pollutants in soil. Results from the WST-1 test are in accordance with our previous samples using Allium cepa (Koynova et al. 2017, Koleva et al. 2018). Other authors also have found a good correlation between tests with human/animal and plant test objects (Grant 1978, Fiskesjö 1993, Tedesco and Laughinghouse 2012, Palmieri et al. 2016, dos Reis et al. 2017).
CONCLUSION
The WST-1 assay can be used for biomonitoring soil quality. The study results show the presence of harmful water-soluble compounds in soils from green spaces for outdoor recreation in Nature Park Shumen Plateau and Shumen city park (Bulgaria). This observation revealed a risk for ecosystems and human health.