SciELO - Scientific Electronic Library Online

 
vol.37 número3Regionalización ecológica de las colonias reproductivas de Zalophus californianus, como herramienta para su conservación en el Golfo de California índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Ciencias marinas

versão impressa ISSN 0185-3880

Resumo

SANCHEZ-RODRIGUEZ, J  e  LUCIO-MARTINEZ, NL. Isolation and prepurification of active compounds in venom from Pelagia noctiluca (Scyphozoa: Pelagiidae) from the Caribbean Sea. Cienc. mar [online]. 2011, vol.37, n.3, pp.369-377. ISSN 0185-3880.

Crude extract from Pelagia noctiluca was purified using liquid chromatography. The isolated fractions were tested in sea crabs (Ocypode quadrata) and neurotoxic activity was observed. The protein content of the crude extract, quantified by the Bradford method, was 0.25 mg mL-1. Polyacrylamide gel electrophoresis was used to determine the apparent molecular weight of the different fractions of the crude extract, and several bands were identified: 34, 48, and between 90 and 200 kDa. Haemolytic activity in human blood type A+ was 100% with 1.1 µg mL-1 and 50% with 0.98 µg mL-1 of crude extract. The crude extract was observed under a microscope and three types of nematocysts were found: heterotrichous microbasic eurytele, heterotrichous isorhiza, and holotrichous isorhiza.

Palavras-chave : isolation; Pelagia nuctiluca; venom; Caribbean Sea.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons