Resumo

HIEBERT, Mickel R. et al. Antimycobacterial activity of the ethanolic extract of the wood of Bulnesia Sarmientoi Lorentz Ex. Griseb.. Rev. latinoam. quím [online]. 2012, vol.40, n.1, pp.7-12. ISSN 0370-5943.

In this work the in vitro antimycobacterial activity of the ethanolic extract obtained from the wood of Bulnesia sarmientoi Lorentz ex. Griseb and its chemical composition were studied. The preliminary phytochemical analysis of the extract showed the presence of steroids and/or triterpenes and tannins, and a GC/MS fingerprint analysis identified guaiol and bulnesol as main volatile components of the extract, based on their retention index and the comparison of their mass spectra with those contained in NIST libraries. The extract showed activity against Mycobacterium tuberculosis H37Rv strain with a minimal inhibitory concentration (MIC) of 50 µg/mL, using the microplate Alamar Blue assay. These results suggest that the extract could be a source of antimicrobial substances against M. tuberculosis.

Palavras-chave : Bulnesia sarmientoi; antimycobacterial; Alamar Blue; Mycobacterium tuberculosis H37Rv; GC/MS.