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Boletín médico del Hospital Infantil de México
versão impressa ISSN 1665-1146
Resumo
PARRA-ORTEGA, Israel et al. Detection and quantification of T-cell subpopulations and NK cells in peripheral blood from healthy individuals. Bol. Med. Hosp. Infant. Mex. [online]. 2019, vol.76, n.2, pp.66-78. ISSN 1665-1146. https://doi.org/10.24875/bmhim.18000083.
Background:
The knowledge of the participation of different subpopulations of T lymphocytes in various pathologies helps to make therapeutic decisions, having as reference the presence of the different subpopulations of the T lymphocytes associated with the disease.
Methods:
A profile standardization of surface molecules for the characterization of subpopulations of T cells was conducted: naïve, activated and memory, as well as natural killer (CD3− CD56+) cells in peripheral blood of clinically healthy individuals.
Results:
Naïve (CD3+, CD4+ or CD8+, CD45RA+, CD62L+, CCR7+), activated (CD3+, CD4+ or CD8+, CD45RA+ or CD45RO+, CD69+ and/or CRTAM+), effectors (CD3+, CD4+ o CD8+, CD45RA+, CD62L−, CCR7−), central memory (CD3+, CD4+ o CD8+, CD45RO+, CD62L+, CCR7+), memory effectors (CD3+, CD4+ or CD8+, CD62RO+, CD62L−, CCR7−) subpopulations were analyzed by flow cytometry. Descriptive statistics parameters were calculated (minimum values, maximum values, mean values, median).
Conclusions:
This panel can be very useful for monitoring patients in whom the immunological status from a cellular perspective is needed. Particularly, it can support the follow-up of patients who require an immunological reconstitution (T-cell component) evaluation.
Palavras-chave : T lymphocyte; Lymphocyte subpopulation; Flow cytometry.