Resumo

CORONA MARTINEZ, José Daniel; PEREZ SOTO, Elvia  e  SANCHEZ MONROY, Virginia. Molecular identification of bacteria in healthy and diseased periodontium. Rev. Odont. Mex [online]. 2019, vol.23, n.1, pp.23-30. ISSN 1870-199X.

The study of bacterial microbiota associated with periodontitis is essential to develop effective diagnostic tools and therapies. Hence, the aim of this study was to identify the bacteria associated with periodontitis. We used 16S rRNA gene amplification by polymerase chain reaction (PCR) and sequencing to identify and compare 80 subgingival samples from patients with healthy periodontium and patients with severe and moderate periodontitis. We identified the following bacteria: Stenotrophomonas maltophilia, Porphyromonas gingivalis, Pseudomonas sp., Fusobacterium nucleatum, Haemophilus sp., Aggregatibacter sp., and Prevotella intermedia. P. gingivalis and F. nucleatum were associated with both moderate and severe periodontitis, and Aggregatibacter sp. was associated with severe periodontitis. The results of this research can be useful in clinical decision-making for treatment of patients with periodontitis.

Palavras-chave : Periodontitis; PCR; sequencing; 16S ribosomal RNA.

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