Resumo

LIRA-MORALES, J. D. et al. SARS-CoV-2 RNA decay on raw environmental aquatic matrices. Revista bio ciencias [online]. 2023, vol.10, e1529.  Epub 12-Abr-2024. ISSN 2007-3380.  https://doi.org/10.15741/revbio.10.e1529.

SARS-CoV-2 is present in corporal fluids of individuals with symptomatic or asymptomatic infections, these fluids are deposited on different water matrices, including drainage water; therefore, wastewater surveillance of RNA of SARS-CoV-2 has emerged as a promising tool as an early warning of potential outbreaks. It is known that the viral genetic material is present in wastewater for several days. However, more data on SARS-CoV-2 is needed to better understand the viral load kinetics in raw water matrices. This work aimed to assess the viral load decay of SARS-CoV-2 RNA in different raw aquatic matrices to evaluate the RNA stability through time. Each water matrix (seawater, influent wastewater, effluent wastewater, and tap water) was inoculated and evaluated for 62 days to determine the viral load kinetic decay by RT-PCR in real-time. SARS-CoV-2 RNA was detected in all water matrices during the experiment. Effluent, influent, and seawater water matrices harsh conditions constraint SARS-CoV-2 RNA detection, with a half-life of 15.24, 43.24, and 32.38 days, and T90 values of 50.63, 143.64, and 107.54 days respectively. Meanwhile, in tap water, the viral genetic material remained for the longest time without significant changes. This study successfully demonstrates that the viral load is affected by the physicochemical characteristics of the water matrix yet confirms that the surveillance of recreational waters and wastewater for SARS-CoV-2 can be a valuable tool for WBE (Wastewater-based epidemiology) as a leading indicator of changes in COVID-19 burden in a community.

Palavras-chave : SARS-CoV-2; water; viral load kinetics; genomic surveillance; environment.

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