Resumo

CRUZ-VALENCIA, Cuauhtémoc et al. Decapacitating effect of oviductal proteins in rooster spermatozoa in vitro. Abanico vet [online]. 2023, vol.13, e106.  Epub 27-Out-2023. ISSN 2448-6132.  https://doi.org/10.21929/abavet2023.13.

In birds, the process of sperm maturation has not been fully described. The objective of this study was to determine in vitro parameters for the decapacitating effect of oviductal proteins on rooster spermatozoa. Aliquots with spermatozoon were incubated to in vitro induce metabolic states: capacitation, decapacitation, and the acrosomal reaction to again determined percentages of live spermatozoa, motility, and concentrations of Malondialdehyde, reduced Glutathione and Adenosine triphosphate. The percentages of motility and live sperm in fresh and capacitated semen were similar (P>0.05), but higher (P<0.05) than those determined in decapacitated semen and with acrosome reaction. The determination of nmol/ml of MDA in fresh semen (1.59) was higher (P<0.005) than in capacitated semen (1.05), with acrosomal reaction (1.07) and decapacitated semen (1.05). GSH nmol/ml were similar (P>0.05) in fresh (72.6) and capacitated (62.04) semen, similarly (P<0.05) between acrosome reaction (99.09) and decapacitated (86.07) semen. The highest concentration of ATP was in capacitated semen with 69.9 µmol/ml, with similar concentrations (P<0.05) in fresh, with acrosomal reaction, and decapacitated semen. The parameters, determined, demonstrated that the protein fraction of the utero-vaginal junction, in vitro, produces decapacitation and successfully maintains sperm viability.

Palavras-chave : adenosine triphosphate; reduced glutathione; malondialdehyde; oxidation; semen.

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