Servicios Personalizados
Revista
Articulo
Indicadores
- Citado por SciELO
- Accesos
Links relacionados
- Similares en SciELO
Compartir
Revista mexicana de ingeniería química
versión impresa ISSN 1665-2738
Rev. Mex. Ing. Quím vol.12 no.3 Ciudad de México dic. 2013
Biotecnología
Efecto de la temperatura y de dos procesos de secado sobre la actividad insecticida de un nucleopoliedrovirus de Spodoptera frugiperda
Effect of temperature and two drying processes on the insecticidal activity of a nucleopolyhedrovirus of Spodoptera frugiperda
J.E. Camacho1,2*, M.I. Gómez3 y L.F. Villamizar3
1 Doctorado en Biociencias, Universidad de la Sabana. Puente del Común Km 7 Auto Norte, Bogotá, Colombia. * Autora para la correspondencia. E-mail: jelenacamacho@hotmail.com.
2 Universidad Colegio Mayor de Cundinamarca. Calle 28 No 6 - 02, Bogotá, Colombia.
3 Centro de Biotecnología y Bioindustria, Corpoica. Km 14 vía Mosquera, Bogotá, Colombia.
Recibido 9 de Mayo de 2013
Aceptado 2 de Julio de 2013
Resumen
La efectividad y uso potencial de los Baculovirus para el control de diferentes insectos plaga han sido demostrados en varios estudios. Sin embargo, su evaluación bajo condiciones de campo ha presentado resultados inconsistentes, posiblemente por el efecto deletéreo de condiciones ambientales como la temperatura. Teniendo en cuenta el potencial del aislamiento colombiano de nucleopoliedrovirus SfMNPV003 de Spodoptera frugiperda para el desarrollo de un bioplaguicida, el objetivo del presente estudio fue evaluar el efecto de la temperatura y de dos procesos de secado sobre la actividad insecticida del virus, con miras a utilizar condiciones del proceso que permitan diseñar una formulación eficiente. Ej nucleopoliedrovirus, fue expuesto a temperaturas entre 30oC y 60oC durante 30, 45, 60, 120 y 180 minutos. Los procesos ensayados fueron secado en estufa y atomización en lecho fluido, evaluandose el pH, el contenido de humedad, el contenido de contaminantes, la concentración viral, el rendimiento y la actividad insecticida (CL50) del virus seco. La actividad insecticida del virus disminuyó cuando éste se expuso a temperaturas mayores a 50°C. El proceso de secado en estufa fue seleccionado por presentar mayor rendimiento, además porque no afectó la patogenicidad del virus y permitió obtener un polvo seco con menor contenido de contaminantes microbianos, sin embargo su consumo energético podría ser mayor.
Palabras clave: baculovirus, temperatura, atomización en lecho fluido, secado en estufa, actividad insecticida.
Abstract
The effectiveness and potential use of Baculovirus for controlling different insect pests have been shown in several studies. However, their evaluation under field conditions has presented inconsistent results, possibly because of the deleterious effect of environmental conditions such as temperature. Considering the potential of a Colombian isolate of Spodoptera frugiperda nucleopolyhedrovirus SfMNPV003 for developing a biopesticide, the objective of this study was to evaluate the effect of temperature and two drying processes over viral insecticidal activity, in order to use process conditions that allow to design efficient formulation. The nucleopolyhedrovirus, was exposed to temperatures between 30°C and 60°C for 30,45,60, 120 and 180 minutes. Processes tested were oven drying and spray drying in fluid bed, evaluating pH, moisture content, content of microbial contaminants, viral concentration, yield and insecticidal activity (LC50) of dry virus. The insecticidal activity of the virus decreased when it was exposed to temperatures above 50°C. Oven drying process was selected for presenting the highest yield, also because it did not affect the virus pathogenicity and allowed to obtain a dry powder with a lower microbial contaminants content.
Keywords: baculovirus, temperature, spray drying in fluid bed, oven drying, insecticidal activity.
DESCARGAR ARTÍCULO EN FORMATO PDF
Referencias
Amorij, J., Huckriede, A., Wilschut, J., Frijlink, H. y Hinrichs, W. (2008). Development of Stable Influenza Vaccine Powder Formulations: Challenges and Possibilities. Pharmaceutical Research 25, 1256-1273. [ Links ]
Arthurs, S., Lacey, L. y Behle, R. (2006). Evaluation of spray dried lignin-based formulations and adjuvants as solar protectans for the granulovirus of the codling moth, Cydia pomonella (L). Journal of Invertebrate Pathology 93, 88-95 [ Links ]
Batista, A., Alves, S., Augusto, N., Pereira, R. y Alves, L. (2001). Stability and persistence of two formulations containig Anticarsia gemmatalis Nuclear polyhedrovirus (AgMNPV). Neotropical Entomology 30, 411-416. [ Links ]
Behle, R. y Popham, H. (2012). Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda. Journal of Invertebrate Pathology 109, 194-200. [ Links ]
Behle, R., Tamez-Guerra, P. y Mcguire, M. (2006). Evaluating conditions for producing spray-dried formulations of Anagraphafalcifera nucleopolyhedroviruses (AgMNPV). Biocontrol Science and Technology 16, 941-952 [ Links ]
Behle, R., Tamez-Guerra, P. y Mcguire, M. (2000) . Effect of Light Energy on Alkali-Released Virions from Anagrapha fa/cifera Nucleopolyhedrovirus. Journal of Invertebrate Pathology 76, 120-126. [ Links ]
Boschetti, N., Wyss, K., Mischler, A., Hostettler, T. y Kempf, C. (2003). Stability of minute virus of mice against temperature and sodium hydroxide. Biologicals 31, 181-185. [ Links ]
Burges, H. (1981). Microbial Control of pest and plant diseases 1970-1980. Academic Press. London. [ Links ]
Burges, H. (1998). Formulation of microbial biopesticides: Beneficial microorganisms, nematodes and seed treatments. Kluwer Academic Publishers. Holanda. [ Links ]
Bürki, K., Jeon, I., Arpagaus, C. y Betz, G. (2011). New insights into respirable protein powder preparation using a nano spray dryer. International Journal of Pharmaceutics 408, 248-256. [ Links ]
Caballero, P., Lopez Ferber, M. y Williams, T. (2001) . Los baculovirus y sus aplicaciones como bioinsecticidas en el control biológico de plagas. Universidad pública de Navarra. Editorial Phytoma, España. [ Links ]
Caballero, P., Murillo, R., Muñoz, D. y Williams, T. (2009). El nucleopoliedrovirus de Spodoptera exigua (Lepidoptera: Noctuidae) como bioplaguicida: análisis de avances recientes en España. Revista Colombiana de Entomología 35, 105-115. [ Links ]
Carrillo,H., Gonzalez, D. H., Cruz, J., Barrera, J. F., Roman, A. y Perez, C. (2011). Storage stability and physicochemical properties of passion fruit juice microcapsules by spray-drying. Revista Mexicana de Ingeniería Química 10, 421-430. [ Links ]
Casas, N. y Gáez G. (2011). Cambios morfométricos y de calidad por aplicación de tres fuentes de calcio bajo tratamiento térmico suave en melón (Cucumis melo L.) fresco precortado. Revista Mexicana de Ingeniería Química 10, 431-444. [ Links ]
Finney, D. J. (1952). Probit Analysis. Cambridge University Press. Inglaterra. [ Links ]
Fernandez-Sandoval, M.T., Ortiz-Garcia, M., Galindo, E. y Serrano-Carreon, L. (2012). Cellular damage during drying and storage of Trichoderma harzianum spores. Process Biochemistry 47,186-194. [ Links ]
Gómez, J., Guevara, E.J., Barrera, G.P., Cotes, A.M. y Villamizar, L.F. (2010). Aislamiento, Identificación y Caracterización de Nucleopoliedrovirus Nativos de Spodoptera frugiperda en Colombia. Revista Facultad Nacional de Agronomía de Medellín 63, 5511-5520. [ Links ]
Guimarães, F.R. (2003). Patogenicidade do nucleopolyhedrovirus (NPV) (Baculoviridae) isolados em larvas de Spodoptera exigua e S. littoralis sobre larvas de S. frugiperda. Manejo integrado de plagas y agroecología (Costa Rica) 70,74-77. [ Links ]
Horaczek, A. y Viernstein, H. (2004). Comparison of three commonly used drying technologies with respect to activity and longevity of aerial conidia of Beauveria brongniartii and Metarhizium anisopliae. Biological Control 31, 65-71. [ Links ]
Hughes, P. y Wood, H. A. (1981). A synchronous per oral technique for the bioassay of insect viruses. Journal of Invertebrate Pathology 37, 154-159. [ Links ]
Jin, X. y Custis, D. (2011). Microencapsulating aerial conidia of Trichoderma harzianum through spray drying at elevated temperatures. Biological Control 56, 202-208. [ Links ]
Johansen, P.; Merkle, H.; Gander, B. (2000). Technological considerations related to the up-scaling of protein microencapsulation by spray-drying. European Journal ofPharmaceutics and Biopharmaceutics 50, 413-417. [ Links ]
Jenkins, N. y Grzywacz, D. (2003). Towards the standardization of Quality Control of fungal and viral Biocontrol Agents. En: Quality Control and Production of Biological Control Agents. Theory and Testing Procedures. (ed. J.C. Van Lenteren). Pp 247-263 .Wageningen University, Netherlands. [ Links ]
Jenkins, N. y Grzywacz, D. (2000). Quality control of fungal and viral biocontrol agents- Assurance of product performance. Biocontrol Science and Technology 10, 753-777. [ Links ]
Lacey, L. y Arthurs, S. (2005). New method for testing solar sensitivity of commercial formulations of the granulovirus of codling moth (Cydia pomonella, Tortricidae: Lepidoptera). Journal of Invertebrate Pathology 90, 85-90. [ Links ]
Lacey L. y Kaya H.K. (2007). Field Manual of Techniques in Invertebrate Pathology. Application and Evaluation of Pathogens for Control of Insects and other Invertebrate Pest. 2a. Edicioín. Springer, Holanda. [ Links ]
Lasa, R., Williams, T. y Caballero, P. (2008). Insecticidal Properties and Microbial Contaminants in a Spodoptera exigua Multiple Nucleopolyhedrovirus (Baculoviridae) Formulation Stored at Different Temperatures. Journal of Economy Entomology 101, 42-49. [ Links ]
Lasa, R., Ruiz, C., Alcazar, M., Belda, J., Caballero, P. y Williams, T.( 2007). Efficacy of optical brightener formulations of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) as a biological insecticide in greenhouses in southern Spain. Biological Control 40, 89-96. [ Links ]
Lian, W., Hsiao, H. y Chou, Ch. (2002). Survival of bifidobacteria after spray-drying. International Journal of Food Microbiology 74, 79-86. [ Links ]
Martínez, A., Williams, T., Loípez, M.F. y Caballero, P. (2005). Optical Brighteners Do Not Influence Covert Baculovirus Infection of Spodoptera frugiperda. Applied and Environmental Microbiology 71,1668-1670. [ Links ]
Michalsky, R., Pfromm, P., Czermak, P., Sorensen, C. y Passarelli, A.L. (2008). Effects of temperature and shear force on infectivity of the baculovirus Autographa californica nucleopolyhedrovirus. Journal of Virological Methods 153, 90-96. [ Links ]
Mille, Y., Girard, J., Beney, L. y Gervais, P. (2005). Air drying optimization of Saccharomyces cerevisiae through its water-glycerol dehydration properties. Journal of Applied Microbiology 99, 376-382. [ Links ]
Miller, L.K. (1997). The Baculoviruses. PLENUM PRESS, New York, London. [ Links ]
Morgan, C., Herman, N., White, P.A. y Vesey, G. (2006). Preservation of micro-organisms by drying: A review. Journal of Microbiological Methods 66, 183-193. [ Links ]
Moscardi, F. (1999). Assessment of the application of baculoviruses for control of lepidoptera. Annual Review Entomology 44, 257-289. [ Links ]
Ohtake, S., Martin, R., Yee, L., Chen, D., Kristensen, D., Lechuga-Ballesteros, D. y Truong-Le, V. (2010) . Heat-stable measles vaccine produced by spray drying. Vaccine 28, 1275-1284. [ Links ]
Parikh, M. D. (2007). Handbook of Pharmaceutical Granulation Technology. Second Edition. Informa Health Care, New York. [ Links ]
Peng, F., Fuxa, J.R., Richter, A. y Johnson, S. (1999). Effects of Heat-Sensitive Agents, Soil Type, Moisture, and Leaf Surface on Persistence of Anticarsia gemmatalis (Lepidoptera: Noctuidae) Nucleopolyhedrovirus. Environmental Entomology 28, 330-338. [ Links ]
Peighambardoust, S., Golshan, A. y Hesari, J. (2011). Application of spray drying for preservation of lactic acid starter cultures: A review. Trends in Food Science & Technology 22, 215-224. [ Links ]
Quiroga, I., Gómez, A. M., Villamizar, L. (2011) . Estabilidad de formulaciones a base de granulovirus para controlar Tecia solanivora (Lepidoptera: Gelechiidae) en campo. Revista Colombiana de Entomología 37, 27-35. [ Links ]
Roberts, P., More, J., Rott, J. y Lewin, D. (2011). Virus inactivation in albumin by a combination of alkali conditions and high temperature. Biologicals 39, 67-72. [ Links ]
Salazar, R. (2003). Tecnología Farmacéutica Industrial. Fabricación y control de medicamentos sólidos de administración por vía oral. Labortecnic, Barcelona. [ Links ]
Simón, O., Williams, T., López, M. y Caballero, P. (2004). Genetic Structure of a Spodoptera frugiperda Nucleopolyhedrovirus Population: High Prevalence of Deletion Genotypes. Applied and Environmental Microbiology 70, 5579-5588. [ Links ]
Silva, M. y Moscardi, F. (2002). Field Efficacy of the Nucleopolyhedrovirus of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae): Effect of Formulations, Water pH, Volume and Time of Application, and Type of Spray Nozzle. Neotropical Entomology 31, 075-083. [ Links ]
Sofer, G., Lister, D. y Boose, J. (2003). Virus Inactivation in the 1990s and into the 21st Century Part 6, Inactivation Methods Grouped by Virus. BioPharm International 42-68. [ Links ]
Sporleder, M., Zegarra, O., Rodriguez, E.M. y Kroschel, J. (2008). Effects of temperature on the activity and kinetics of the granulovirus infecting the potato tuber moth Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae). Biological Control 44, 286-295. [ Links ]
Tamez, P., Zamudio, V., Carrillo, J., Rodríguez, C. y Gómez, R. (2006). Formulaciones granulares de Baculovirus en combinación con abrillantadores ópticos para su empleo como Bioinsecticida. Ciencia UANL 9, 149-156. [ Links ]
Tamez, P., Mcguire, M., Behle, R., Shasha, B. y PingeL, R. (2002). Storage Stability of Anagrapha falcifera nucleopolyehedrovirus in spray dried formulations. Journal of Invertebrate Pathology 79, 7-16. [ Links ]
Valicente, F. y Da costa E. (1995). Controle da lagarta do cartucho, Spodoptera frugiperda (J.E. Smith), como Baculovirus spodoptera, aplicado via água de irrigação. Annual Society Entomology Brasil 24, 61-67. [ Links ]
Villamizar, L. F. (2011). Virus entomopatógenos y cambio climático. 127-143p. En: Ed: Sociedad Colombiana De Entomología Socolen. Memorias Cambio Climático: Retos Y Oportunidades Para La Entomología. 38 Congreso Socolen. Colombia. [ Links ]
Villamizar, L., Barrera, G., Cotes, A. y Martínez, F. (2010). Eudragit S100 microparticles containing Spodoptera frugiperda nucleopolyehedrovirus: Physicochemical, characterization, photostability and in vitro virus release. Journal of Microencapsulation 27, 314-324. [ Links ]
Villamizar, L., Espinel, C. y Cotes, A. (2009). Efecto de la radiación ultravioleta sobre la actividad insecticida de un nucleopoliedrovirus de Spodoptera frugiperda (Lepidoptera: Noctuidae). Revista Colombiana de Entomología 35, 116-121. [ Links ]
Viveros, R., Tellez, D. I., Perea, M.J., Alamilla, L., Cornejo, M., Beristain, C. I., Azuara E. y Gutierrez G.F. (2013). Encapsulation of ascorbic acid into calcium alginate matrices through coacervation coupled to freeze-drying. Revista Mexicana de Ingeniería Química 12, 29-39. [ Links ]
Williams, T., Goulson, D., Caballero, P., Cisneros, J., Martínez, A., Chapman, J., Roman, D. y Cave, R. (1999). Evaluation of a baculovirus bioinsecticide for small-scale maize growers in Latin America. Biological Control 14, 67-75. [ Links ]
Winder, R. S., Wheeler, J. J., Conder, N., Otvos, I. S., Nevill, R. y Duan, L. (2003). Microencapsulation: A Strategy for Formulation of Inoculum. Biocontrol Science and Technology 13,155-169. [ Links ]
Young, S. (2005). Persistence of viruses in environment. Disponible en: http://www.Agctr.isu.edu/s265/young.htm. [Fecha revision: 17 febrero 2012] [ Links ].
Zar, J. (1999). Biostatical analysis. 4th ed. Prentice Hall, New Jersey. [ Links ]