Servicios Personalizados
Revista
Articulo
nueva página del texto (beta)
Español (pdf)
Artículo en XML
Referencias del artículo
Traducción automática
Enviar artículo por emailIndicadores
-
Citado por SciELO -
Accesos
Links relacionados
-
Similares en
SciELO
Compartir
Permalink
Revista odontológica mexicana
versión impresa ISSN 1870-199X
Rev. Odont. Mex vol.13 no.4 Ciudad de México dic. 2009
https://doi.org/10.22201/fo.1870199xp.2009.13.4.15558
Trabajos originales
Efecto antimicrobiano del agua potencialmente oxidativa
Antimicrobial effect of the oxidative potential water
*Alumno del 2do año de la Maestría en Endodoncia de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
§Jefa del Laboratorio de Investigación de la Maestría en Endodoncia de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
IIProfesor-Investigador del Instituto de Metalurgia. Universidad Autónoma de San Luis Potosí.
¶Coordinador de la Maestría en Endodoncia de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
**Profesor-Investigador de la Facultad de Estomatología. Universidad Autónoma de San Luis Potosí.
El agua potencialmente oxidativa (APO) es una solución activada por un proceso electrolítico que tiene aplicaciones en el área de la salud.
Objetivo
El objetivo del estudio fue evaluar el efecto antimicrobiano del APO en concentraciones de NaCl al 1% y al 2.5% contra cepas de Enterococcus faecalis aisladas de conducto radicular.
Metodología
Se utilizaron cepas de E. faecalis aisladas de conducto radicular de pacientes con indicación de retratamiento, con presencia de lesión periapical y diagnóstico de periodontitis apical crónica. Se utilizó tioglicolato-prerreducido (BD BBL) como medio de transporte y como medio de cultivo cajas de agar sangre CDC (BD BBL) en condiciones de anaerobiosis. Para evaluar la solución irrigante se utilizó la técnica de dilución serial. Se dividieron 86 muestras en 4 grupos experimentales de APO (1% y 2.5%), con un esquema de irrigación de 9 mL de APO + 1 mL de inóculo con un tiempo de exposición al irrigante de 5 minutos. Los tiempos de incubación para todas las muestras fueron de 24 y 48 h. Como control positivo se utilizó NaOCl 5.25% como irrigante y en el control negativo agua destilada.
Resultados
Se demostró efectividad del APO en concentración de NaCl (Fermont) al 1 y 2.5%, obteniendo un promedio de 28 unidades formadoras de colonias (UFC) y de 2 UFC para el APO al 1 y al 2.5%, respectivamente.
Conclusiones
El APO tiene mayor efecto antimicrobiano en ambas concentraciones de NaCl utilizadas (1 y 2.5%) en comparación con el control. Sin embargo, es necesario realizar estudios que evalúen la eficacia de éste sobre el biofilm bacteriano, así como su biocompatibilidad para proponer su uso como irrigante intraconducto.
Palabras clave: Agua potencialmente oxidativa; E. faecalis; efecto antimicrobiano
The oxidative potential water (OPW) is an electro-chemically activated solution and it is widely used in health fields.
Objective
The objective was to evaluate the antimicrobial effect of the OPW in concentration of 1% and 2.5% of NaCl against E. faecalis isolated from root canal.
Methodology
E. faecalis samples isolated from patients root canals retreatment with an apical lesion and diagnosis of chronic apical periodontitis were used. The samples were incubated in anaerobic conditions in CDC blood agar medium until their use. The serial CDC technique was used to evaluate the root canal irrigant. Eighty six samples were divided in 4 experimental groups of OPW (1% and 2.5%), with an irrigation scheme of 9 mL of OPW + 1 mL of inoculate and with 5 minutes of exposition to the irrigant. The incubation times for all samples were of 24 and 48 hours. In the positive control, 5.25% NaOCl was used and in the negative control, distilled water.
Results
The OPW was effective in concentrations of 1% and 2.5% NaCl, obtaining an average of 28 CFU and 2 CFU for 1% and 2.5% OPW, respectively.
Conclusions
The OPW has an antimicrobial effect in the concentrations used of NaCl (1% and 2.5%). Nevertheless, it is necessary to study the efficacy of this irrigant on the bacterial biofilm and its biocompatibility in order to propose it, as a root canal irrigant.
Key words: Oxidative potential water; E. faecalis; antimicrobial effect
Referencias
1. Marais JT, Williams WP. Antimicrobial effectiveness of electrochemically activated water as an endodontic irrigation solution. Int Endod J 2001; 34(3): 23-43. [ Links ]
2. Hata G, Hayami S, Weine FS, Toda T. Effectiveness of oxidative potential water as a root canal irrigant. Int Endod J 2001; 34(4): 308-317. [ Links ]
3. Gulabivala K, Stock JR, Lewsey JD, Ghori S, Ng YL, Spratt DA. Effectiveness of electrochemically activated water as an irrigant in an infected tooth model. Int Endod J 2004; 37(9): 624-631. [ Links ]
4. Prince EL, Muir AV, Thomas WM, Stollard RJ, Sampson M, Lewis JA. An evaluation of the efficacy of aqualox for microbiological control of industrial cooling tower systems. J Hosp Infect 2002; 52(4): 243-249. [ Links ]
5. Park H, Hung YC, Chung D. Effects of chlorine and pH on efficacy of electrolyzed water for inactivating Escherichia coli 0157: H7 and Listeria monocytogenes. Int J Food Microbiol 2004; 91(1): 13-18. [ Links ]
6. Kohno S, Kawata T, Kaku M, Fujita T, Tsutsui K, Ohtani J, Tenjo K, Motokawa M, Tohma Y, Shigekawa M, Kamata H, Tanne K. Bactericidal effects of acidic electrolyzed water on the dental unit waterline. Jpn J Infect Dis 2004; 57: 52-54. [ Links ]
7. Miyamoto M, Inoue K, Gu Y, Hoki M, Haji S, Ohyanagi H. Effectiveness of acidic oxidative potential water in preventing bacterial infection in islet transplantation. Cell Transplant 1999; 8(4): 405-411. [ Links ]
8. Zehnder M. Root canal irrigants. J Endod 2006; 32(5): 389-398. [ Links ]
9. Estrela C. Ciencia Endodóntica. Artes Médicas, 2005. [ Links ]
10. Silva-Herzog D, Andrade V, Gaona RC, Gaitán C, Trancoso R, Turner K, Sayao S. Propriedades físico-químicas da água potencialmente oxidativa e sua capacidade de remoção da camada residual de instrumentação. Revista Sul-Brasileira de Odontología 2006; 3: 57-61. [ Links ]
11. Becking AG. Complications in the use of sodium hypochlorite during endodontics treatment. Report of three cases. Oral Surg, Oral Med, Oral Path 1991; 71(3): 346-348. [ Links ]
12. Marais JT. Cleaning efficacy of new root canal irrigation: A preliminary evaluation. Int Endod J 2000; 33(4): 320-325. [ Links ]
13. Manzur A, González AM, Pozos A, Silva-Herzog D, Friedman S. Bacterial quantification in teeth with apical periodontitis related to instrumentation and different intracanal medications: a randomized clinical trial. J Endod 2007; 33(2): 114-118. [ Links ]
14. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32(2): 93-98. [ Links ]
15. Kayaoglu G, Ørstavik D. Virulence factors of Enterococcus faecalis relationship to endodontic a disease. Crit Rev Oral Biol Med 2004; 15(5): 308-320. [ Links ]
Lecturas recomendadas
1. Radcliffe CE, Potouridou L. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontics microorganisms Actinomyces Israeli, A. Naeslundii, Candida Albicans and Enterococcus Faecalis. Int Endod J 2004; 37(7): 438-446. [ Links ]
2. Shurrab MY. Antimicrobial efficiency of some antiseptic products on endodontic microflora isolated from gangrenous pulp tissue. J Contemp Dent Pract 2006; 7(4): 53-62. [ Links ]
3. Da Silva LA, Nelson FP, Faria G, De Souza MC, Ito IY. Bacterial profile in primary teeth with necrotic pulp and periapical lesions. Braz Dent J 2006; 17(2): 144-148. [ Links ]
4. Vianna ME, Horz HP, Gomes BP. In vivo evaluation of microbial reduction after chemo-mechanical preparation of human root canals containing necrotic pulp tissue. Int Endod J 2006; 39(6): 484-492. [ Links ]
5. Trevor V. Suslow. Oxidation-reduction potential (ORP) for water disinfection monitoring, control and documentation. University of California, Division of Agriculture and Natural Resources. 2004 Publication 8149. [ Links ]
6. Iwasawa A, Nakamura Y. Bactericidal effect of acidic electrolyzed water-comparison of chemical acidic sodium hydro-chloride (NaOCl) solution Kansenshogaku Zasshi 1996; 70(9): 915-922. [ Links ]
7. Lee SH, Choi BK. Antibacterial effect of electrolyzed water on oral bacteria. J Microbiol 2006; 44(4): 417-422. [ Links ]
8. Nagamatsu Y, Chen KK. Durability of bactericidal activity in electrolyzed neutral water by storage. Dent Mater J 2002; 21(2): 93-104. [ Links ]
9. Nagamatsu Y, Tajima K, Kakigawa H. Application of electrolyzed acid water to sterilization of denture base part 1. Examination of sterilization effects on resin plate. Dent Mater J 2001; 20(2): 148-155. [ Links ]
10. Sundqvist G. Ecology of the root canal flora. J Endod 1992; 18(9): 427-430. [ Links ]
11. Tronstad L, Titterud PS. The evolving new understanding of endodontic infections. Endodontic Topics 2003; 6(1): 56-77. [ Links ]
12. Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res 1987; 66(8): 1375-1379. [ Links ]
Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons
