Resumen

RAMIREZ-MOSQUEDA, Marco A.; LOPEZ-AGUILAR, Raúl; ORDUNO-CRUZ, Andrés  y  RODRIGUEZ-DEMENEGHI, Marco Vinicio. In vitro conservation of Guarianthe skinneri (Bateman) Dressler & W. E. Higgins for minimal growth. Polibotánica [online]. 2024, n.58, pp.171-180.  Epub 22-Oct-2024. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.58.12.

In vitro conservation techniques are considered methods of medium-term conservation of plant germplasm. The conservation period under this technique is usually 1 to 2 years without using any subculture. In Mexico there are plant genetic resources that are cataloged within the Official Mexican Standard NOM-059-SEMARNAT-2010. These plant species must be safeguarded and conserved as a priority. Guarianthe skinneri (Bateman) Dressler & W. E. Higgins is an orchid that has a flower that attracts the attention of horticulturists and/or collectors as an ornamental plant. The objective of this study was to establish an in vitro conservation protocol for G. skinneri through minimal growth. For this, different concentrations (0, 0.5, 1.0 and 2.0 mg L-1) of abscisic acid (ABA), paclobutrazol (PBZ), ancimidol (ACD) and different concentrations (0, 5, 10 and 20 g L-1) of polyethylene glycol (8000). Subsequently, the regeneration of the preserved material was carried out using MS medium added with 2 mg L-1 of 6-benzylaminopurine (BAP). After 12 months of in vitro conservation, the results showed a reduction in the length of the shoots preserved in MS with 2.0 mg L-1 of PBZ and in ABA 0.5 mg L-1. However, in the PBZ treatment, shoot and root formation increased compared to the ABA treatment. The regeneration of 5.4 shoots/explant was achieved at 2.0 mg L-1 of BAP. As well as a survival of 90% during the acclimatization process. The results of this study can contribute to the conservation work carried out on this species of ornamental importance.

Palabras llave : Conservation; Minimum growth; Ancimidol; Polyethylene glycol; In vitro regeneration.

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